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Colloidal gold. principles, methods and applications / Volume 1 :
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
1989.
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| Colección: | Colloidal gold : principles, methods, and applications ;
v. 1 |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Colloidal Gold: Principles, Methods, and Applications; Copyright Page; Table of Contents; List of Contributors; Preface; Contents of Other Volumes; Chapter 1. The Development and Application of Colloidal Gold as a Microscopic Probe; Introduction; Development of Methodology; Development of Applications; Current Trends; References; Chapter 2. Methods for Synthesis of Colloidal Gold; Introduction; Preparation : General Considerations; Methods for Colloidal Gold Synthesis; Evaluation of the Colloidal Gold Preparation; References
- Chapter 3. Protein A
- Gold and Protein G
- Gold Postembedding Immunoelectron MicroscopyIntroduction; Protein A; Protein G; Colloidal Gold Marker; Protein A
- Gold and Protein G
- Gold Complexes; Tissue Processing; Immunolabeling; Immunocytochemical Controls; Quantitative Evaluation; Double-Labeling Techniques; Amplification; Application of The Protein A
- Gold and Protein G
- Gold Complexes; Conclusions; References; Chapter 4. Preparation and Application of Lectin-Gold Complexes; Introduction; The Lectins: Properties and Specificity; Preparation of Lectin
- Gold Complexes
- Cytochemical LabelingApplications to Transmission Electron Microscopy; Conclusions; References; Chapter 5. Colloidal Gold for Multiple Staining; Introduction; Staining Methodology; Multiple Immunostaining Procedures in the Literature; Specificity Tests; Practical Description of Multiple Staining Methods; Appendix; References; Chapter 6. Immunogold Labeling of Ultrathin Cryosections; Introduction; Specimen Preparation; Cryoultramicrotomy; Immunolabeling; Conclusions; References; Chapter 7. Colloidal Gold for Scanning Electron Microscopy; Introduction; Preparation of Colloidal Gold
- Labeling of Colloidal GoldPreparation of Specimens; Marking of Specimens; Coating of Marked Specimens; Viewing of Marked Specimens; Control Experiments; Steric Hindrance; Conclusion; References; Chapter 8. Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces; Introduction; Colloidal Gold Labeling of Cell Surfaces; Choice of an Identifier for Surface-Exposed Target Molecules; Choice of a Ligand for Optimum Gold Labeling; Choice of Colloidal Gold Particle Size; Operation of the Scanning Electron Microscope in the BEI Mode; Application of Silver Enhancement Procedures
- Double LabelingPossible Significance of Counting Gold Markers on Cell Surfaces; Concluding Remarks; References; Chapter 9. Silver-Enhanced Colloidal Gold Method; Introduction; History; Approaches for Silver Enlargement of Colloidal Gold Particles; Criteria of Reliability; Criteria of Practicality; Applications; Conclusions; References; Chapter 10. Silver
- Enhanced Colloidal Gold for Light Microscopy; Introduction; Immunogold Staining; Nonsilver Enhancement Techniques; Silver Enhancement of Colloidal Gold; Immunogold Silver Staining; Indirect Method
- Bridge Methods for Immuno gold
- Silver Staining and Immunogold
- Silver
- PAP Method