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Colloidal gold. principles, methods and applications / Volume 1 :
Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
1989.
|
| Colección: | Colloidal gold : principles, methods, and applications ;
v. 1 |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Colloidal gold. |n Volume 1 : |b principles, methods and applications / |c ed. M.A. Hayat. |
| 260 | |a San Diego : |b Academic Press, |c 1989. | ||
| 300 | |a 1 online resource (XXVII, 536 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 0 | |a Colloidal gold : principles, methods, and applications ; |v v. 1 | |
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| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 520 | |a Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. | ||
| 588 | 0 | |a Print version record. | |
| 504 | |a Includes bibliographical references at the end of each chapters and index. | ||
| 505 | 0 | |a Front Cover; Colloidal Gold: Principles, Methods, and Applications; Copyright Page; Table of Contents; List of Contributors; Preface; Contents of Other Volumes; Chapter 1. The Development and Application of Colloidal Gold as a Microscopic Probe; Introduction; Development of Methodology; Development of Applications; Current Trends; References; Chapter 2. Methods for Synthesis of Colloidal Gold; Introduction; Preparation : General Considerations; Methods for Colloidal Gold Synthesis; Evaluation of the Colloidal Gold Preparation; References | |
| 505 | 8 | |a Chapter 3. Protein A -- Gold and Protein G -- Gold Postembedding Immunoelectron MicroscopyIntroduction; Protein A; Protein G; Colloidal Gold Marker; Protein A -- Gold and Protein G -- Gold Complexes; Tissue Processing; Immunolabeling; Immunocytochemical Controls; Quantitative Evaluation; Double-Labeling Techniques; Amplification; Application of The Protein A -- Gold and Protein G -- Gold Complexes; Conclusions; References; Chapter 4. Preparation and Application of Lectin-Gold Complexes; Introduction; The Lectins: Properties and Specificity; Preparation of Lectin -- Gold Complexes | |
| 505 | 8 | |a Cytochemical LabelingApplications to Transmission Electron Microscopy; Conclusions; References; Chapter 5. Colloidal Gold for Multiple Staining; Introduction; Staining Methodology; Multiple Immunostaining Procedures in the Literature; Specificity Tests; Practical Description of Multiple Staining Methods; Appendix; References; Chapter 6. Immunogold Labeling of Ultrathin Cryosections; Introduction; Specimen Preparation; Cryoultramicrotomy; Immunolabeling; Conclusions; References; Chapter 7. Colloidal Gold for Scanning Electron Microscopy; Introduction; Preparation of Colloidal Gold | |
| 505 | 8 | |a Labeling of Colloidal GoldPreparation of Specimens; Marking of Specimens; Coating of Marked Specimens; Viewing of Marked Specimens; Control Experiments; Steric Hindrance; Conclusion; References; Chapter 8. Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces; Introduction; Colloidal Gold Labeling of Cell Surfaces; Choice of an Identifier for Surface-Exposed Target Molecules; Choice of a Ligand for Optimum Gold Labeling; Choice of Colloidal Gold Particle Size; Operation of the Scanning Electron Microscope in the BEI Mode; Application of Silver Enhancement Procedures | |
| 505 | 8 | |a Double LabelingPossible Significance of Counting Gold Markers on Cell Surfaces; Concluding Remarks; References; Chapter 9. Silver-Enhanced Colloidal Gold Method; Introduction; History; Approaches for Silver Enlargement of Colloidal Gold Particles; Criteria of Reliability; Criteria of Practicality; Applications; Conclusions; References; Chapter 10. Silver -- Enhanced Colloidal Gold for Light Microscopy; Introduction; Immunogold Staining; Nonsilver Enhancement Techniques; Silver Enhancement of Colloidal Gold; Immunogold Silver Staining; Indirect Method | |
| 505 | 8 | |a Bridge Methods for Immuno gold -- Silver Staining and Immunogold -- Silver -- PAP Method | |
| 546 | |a English. | ||
| 650 | 0 | |a Colloidal gold. | |
| 650 | 2 | |a Gold Colloid |0 (DNLM)D017979 | |
| 650 | 6 | |a Or collo�idal. |0 (CaQQLa)201-0184046 | |
| 650 | 7 | |a Colloidal gold |2 fast |0 (OCoLC)fst00868332 | |
| 650 | 7 | |a Or. |2 fmesh | |
| 650 | 7 | |a Immunohistochimie |x M�ethode. |2 fmesh | |
| 650 | 7 | |a Microscopie �electonique |x M�ethode. |2 fmesh | |
| 650 | 7 | |a Prot�eines |x analyse. |2 fmesh | |
| 700 | 1 | |a Hayat, M. A., |d 1940- | |
| 776 | 0 | 8 | |i Print version: |t Colloidal gold. |d San Diego : Academic Press, 1989 |w (OCoLC)25697574 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780123339270 |z Texto completo |