Guide to techniques in mouse development. Part B, Mouse molecular genetics /

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensivel...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Wassarman, Paul M., Soriano, Philippe M., 1953-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier /Academic Press, 2010.
Edición:1st ed.
Colección:Methods in enzymology ; v. 477.
Temas:
Mice > Cytology.
Mice > Embryology.
Mice > Development.
Cell lines.
Cell Line
Embryonic Stem Cells > cytology
Mice > embryology
Mice > growth & development
Souris > Cytologie.
Souris > Embryologie.
Souris > D�eveloppement.
Lign�ees cellulaires.
SCIENCE > Life Sciences > Cell Biology.
Laboratory manuals.
Laboratory Manual
Manuels de laboratoire.
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • Part B. Lentivirus transgenesis
  • Lentivirus transgenesis
  • Germline modification using mouse spermatogonial stem cells
  • Embryonic in vivo electroporation in the mouse
  • Current applications of transposons in mouse genetics
  • Functional genomics in the mouse using the sleeping beauty transposon system
  • The use of DNA transposons for cancer gene discovery in mice
  • A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of creert2
  • A recombineering pipeline to make conditional targeting constructs
  • Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria
  • Genetic fate mapping using site-specific recombinases
  • Mapping cell fate and function using recombinase-based intersectional strategies
  • Genome-wide forward genetic screens in mouse es cells
  • Gene trap mutagenesis in the mouse
  • A wider context for gene trap mutagenesis
  • Mouse mutagenesis with the chemical supermutagen enu
  • Phenotype-driven mouse enu mutagenesis screens
  • Using enu mutagenesis for phenotype-driven analysis of the mouse
  • Exploration of self-renewal and pluripotency in es cells using rnai
  • Transgenic rnai applications in the mouse
  • Gene knockdown in the mouse through rnai
  • In vivo analysis of gene knockdown in tetracycline-inducible shrna mice
  • The power of reversibility regulating gene activities via tetracycline-controlled transcription
  • Gene expression profiling of mouse oocytes and preimplantation embryos
  • Interrogating the transcriptome of oocytes and preimplantation embryos
  • Gene expression profiling of mouse embryos with microarrays
  • Technological advances in mouse development studies.

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