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Cryo-EM. Part A, Sample preparation and data collection /
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, Calif :
Academic Press/Elsevier,
�2010.
|
| Colección: | Methods in enzymology ;
v. 481. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Part A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development
- Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection
- Helical crystallization of soluble and membrane binding proteins
- Plunge freezing for electron cryomicroscopy
- A practical guide to the use of monolayer purification and affinity grids
- Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
- Cryonegative staining of macromolecular assemblies
- Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM
- Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing
- Site-specific biomolecule labeling with gold clusters
- How to operate a cryo-electron microscope
- Collecting electron crystallographic data of two-dimensional protein crystals
- Automated data collection for electron microscopic tomography
- Correlated light and electron cryo-microscopy
- Phase plates for transmission electron microscopy
- Radiation damage in electron cryomicroscopy.