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Cryo-EM. Part C, Analyses, interpretation, and case studies /
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, Calif :
Academic Press/Elsevier,
�2010.
|
| Colección: | Methods in enzymology ;
v. 483. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 020 | |a 0123849934 |q (electronic bk.) | ||
| 035 | |a (OCoLC)668081543 |z (OCoLC)671244756 | ||
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| 060 | 4 | |a QU135 |b .M49 v.483 | |
| 082 | 0 | 4 | |a 502.825 |2 22 |
| 245 | 0 | 0 | |a Cryo-EM. |n Part C, |p Analyses, interpretation, and case studies / |c edited by Grant J. Jensen. |
| 260 | |a San Diego, Calif : |b Academic Press/Elsevier, |c �2010. | ||
| 300 | |a 1 online resource (xlv, 360 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file |2 rda | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 483 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 500 | |a Title from PDF title page (ScienceDirect, viewed Oct. 4, 2010). | ||
| 520 | |a This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide. | ||
| 505 | 0 | |a 1.Introduction / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 2.Features in a Subnanometer Resolution Density Map / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 3.Tools: Analyzing a Subnanometer Resolution Density Map / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 4.Protocol: From Density Map to Atomic Model / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 5.Case Study: Mm-cpn / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 6.Discussion / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- Acknowledgments / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- References / Matthew L. Baker / Mariah R. Baker / Corey F. Hryc / Frank DiMaio -- 1.Introduction / Niels Volkmann -- 2.Noise Reduction / Niels Volkmann -- 3.Segmentation / Niels Volkmann -- 4.Detection and Mapping of Macromolecular Assemblies / Niels Volkmann -- 5.Classification and Averaging / Niels Volkmann -- 6.Validation / Niels Volkmann -- Acknowledgments / Niels Volkmann -- References / Niels Volkmann -- 1.Introduction / Friedrich F�orster / Elizabeth Villa -- 2.The Problem of Placing Assembly Subunits into Cryo-EM Maps / Friedrich F�orster / Elizabeth Villa -- 3.Structure Prediction of Subunits / Friedrich F�orster / Elizabeth Villa -- 4.Protein[-]Protein Interaction Data / Friedrich F�orster / Elizabeth Villa -- 5.Model Building of a Complex Using Cryo-EM and Additional Data / Friedrich F�orster / Elizabeth Villa -- 6.Refinement of Atomic Models Using High-Resolution Maps / Friedrich F�orster / Elizabeth Villa -- 7.Conclusion and Outlook / Friedrich F�orster / Elizabeth Villa -- References / Friedrich F�orster / Elizabeth Villa -- 1.Introduction / Catherine L. Lawson -- 2.EM Structural Data Archives / Catherine L. Lawson -- 3.Deposition and Content / Catherine L. Lawson -- 4.Access / Catherine L. Lawson -- 5.Examples of Use / Catherine L. Lawson -- 6.Future Prospects / Catherine L. Lawson -- 7.Guide to Deposition of EM Structural Data / Catherine L. Lawson -- Acknowledgments / Catherine L. Lawson -- References / Catherine L. Lawson -- 1.Electron Crystallography / Andreas D. Schenk / Richard K. Hite / Andreas Engel / Yoshinori Fujiyoshi / Thomas Walz -- 2.Contributions of Electron Crystallography to the Structural Biology of Aquaporins / Andreas D. Schenk / Richard K. Hite / Andreas Engel / Yoshinori Fujiyoshi / Thomas Walz -- 3.Contributions of Structural Studies on Aquaporins to Advances in Electron Crystallography / Andreas D. Schenk / Richard K. Hite / Andreas Engel / Yoshinori Fujiyoshi / Thomas Walz -- Acknowledgments / Andreas D. Schenk / Richard K. Hite / Andreas Engel / Yoshinori Fujiyoshi / Thomas Walz -- References / Andreas D. Schenk / Richard K. Hite / Andreas Engel / Yoshinori Fujiyoshi / Thomas Walz -- 1.Introduction: The Role of Electron Microscopy in Tubulin Studies / Kenneth H. Downing / Eva Nogales -- 2.Tubulin Studies by Electron Crystallography / Kenneth H. Downing / Eva Nogales -- 3.Microtubule Structure / Kenneth H. Downing / Eva Nogales -- 4.Structure of Microtubule Assembly/Disassembly Intermediates / Kenneth H. Downing / Eva Nogales -- 5.Mechanism of Kinesin Movement Along Microtubule / Kenneth H. Downing / Eva Nogales -- 6.Drug Binding Studied by Diffraction and Modeling / Kenneth H. Downing / Eva Nogales -- 7.Tomography for Larger Structures / Kenneth H. Downing / Eva Nogales -- 8.Conclusion / Kenneth H. Downing / Eva Nogales -- Acknowledgments / Kenneth H. Downing / Eva Nogales -- References / Kenneth H. Downing / Eva Nogales -- 1.Helical Crystallization of the Bacterial Integral Membrane Protein, MsbA / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- 2.Helical Crystallization of the Sarcoplasmic Reticulum Ca2+-ATPase / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- 3.Discussion / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- 4.Conclusions / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- Acknowledgments / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- References / John Paul Glaves / Lauren Fisher / Andrew Ward / Howard S. Young -- 1.Introduction / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- 2.Dealing with Heterogeneity: 3D Sorting / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- 3.Subnanometer Multiparticle Cryo-EM of Ribosomes in Practice / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- 4.Interpretation / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- 5.Conclusions / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- Acknowledgments / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- References / Justus Loerke / Jan Giesebrecht / Christian M.T. Spahn -- 1.Electron Microscopy and the Next Frontier in Structural Biology / Edward J. Brignole / Francisco Asturias -- 2.The Catalytic Cycle of FAS Requires Conformational Changes / Edward J. Brignole / Francisco Asturias -- 3.Methods and Rationale Employed in the Conformational Analysis of FAS / Edward J. Brignole / Francisco Asturias -- 4.EM and FAS: A Versatile Tool for a Flexible Macromolecule / Edward J. Brignole / Francisco Asturias -- Acknowledgments / Edward J. Brignole / Francisco Asturias -- References / Edward J. Brignole / Francisco Asturias -- 1.Introduction / Dorit Hanein -- 2.Testing the Lamella Hypothesis / Dorit Hanein -- 3.Challenges / Dorit Hanein -- Acknowledgments / Dorit Hanein -- References / Dorit Hanein -- 1.Introduction / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 2.Data Acquisition / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 3.Templates / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 4.Template Matching / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 5.Assessment of Performance / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 6.The Spatial Proteome of L. interrogans / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 7.Outlook / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- Acknowledgments / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- References / Friedrich F�orster / Bong-Gyoon Han / Martin Beck -- 1.Introduction / Asaf Mader / Nadav Elad / Ohad Medalia -- 2.Specimen Preparation / Asaf Mader / Nadav Elad / Ohad Medalia -- 3.Relying on Correlative Light and Electron Microscope for Cellular Structural Study of Eukaryotic Cells / Asaf Mader / Ohad Medalia / Nadav Elad -- 4.Cryoelectron Tomography of Cytoskeleton-Driven Processes / Asaf Mader / Nadav Elad / Ohad Medalia -- 5.Cryotomography of Midbodies / Asaf Mader / Nadav Elad / Ohad Medalia -- 6.Structural Analysis of the Nuclear Pore Complex by Cryo-ET / Asaf Mader / Nadav Elad / Ohad Medalia -- 7.Concluding Remarks / Asaf Mader / Nadav Elad / Ohad Medalia -- Acknowledgments / Asaf Mader / Nadav Elad / Ohad Medalia -- References / Asaf Mader / Nadav Elad / Ohad Medalia -- 1.Introduction / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- 2.Cryoelectron Tomography / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- 3.3D Visualization of Intact HIV Virion / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- 4.Conclusions and Perspective / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- Acknowledgments / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- References / Jun Liu / Elizabeth R. Wright / Hanspeter Winkler -- 1.Introduction / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- 2.Specimen Preparation / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- 3.Autoloading and Robotic Screening / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- 4.Microscopy / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. | |
| 505 | 0 | |a Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- 5.Image Processing / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher / Dmitry Lyumkis / Arne Moeller -- 6.Assessment and Integration / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- 7.The Future of Automation / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- Acknowledgments / Dmitry Lyumkis / Arne Moeller / Anchi Cheng / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / James Pulokas / Joel D. Quispe / Neil R. Voss / Clinton S. Potter / Bridget Carragher -- References / Arne Moeller / Dmitry Lyumkis / Bridget Carragher / Clinton S. Potter / Neil R. Voss / Joel D. Quispe / James Pulokas / Amber Herold / Eric Hou / Christopher Irving / Erica L. Jacovetty / Pick-Wei Lau / Anke M. Mulder / Anchi Cheng. | |
| 650 | 0 | |a Electron microscopy |x Technique. | |
| 650 | 0 | |a Proteins |x Structure. | |
| 650 | 0 | |a Genomics. | |
| 650 | 0 | |a Electron microscopy. | |
| 650 | 0 | |a Proteins. | |
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| 700 | 1 | |a Jensen, Grant J. | |
| 776 | 0 | 8 | |i Print version: |t Cryo-EM. Part C, Analyses, interpretation, and case studies. |d London : Academic, 2010 |z 9780123849939 |w (OCoLC)653082346 |
| 830 | 0 | |a Methods in enzymology ; |v v. 483. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780123849939 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/483 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/483 |z Texto completo |