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Introduction to electron microscopy.
Introduction to Electron Microscopy, Second Edition provides an introduction to the foundations of electron microscopy; an outline of some practical aspects of instrument operation; and discussion of the rationale of the methodology of biological specimen preparation. The book seeks to provide a com...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Pergamon Press,
[1970]
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| Edición: | 2d ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Introduction to Electron Microscopy; Copyright Page; Dedication; PREFACE; Table of Contents; Part One: INSTRUMENTATION; Chapter 1. INTRODUCTION; Chapter 2. HISTORICAL REVIEW; Chapter 3. BASIC THEORY OF ELECTRON MICROSCOPY; A. NATURE OF LIGHT BEAMS; B. RESOLUTION; C. NATURE OF ELECTRON BEAMS; D. ELECTRON EMISSION; E. ELECTRON OPTICS; F. ANALOGY BETWEEN THE LIGHT AND ELECTRON MICROSCOPES; Chapter 4. THE ELECTRON MICROSCOPE; A. ILLUMINATION SYSTEM; B. IMAGING SYSTEM; C. IMAGE TRANSLATING SYSTEM; D. OTHER ELECTRON MICROSCOPE COMPONENTS; E. OPERATIONAL REQUIREMENTS.
- F. OPERATION OF THE ELECTRON MICROSCOPEG. ACCESSORIES FOR THE ELECTRON MICROSCOPE; H. OTHER TYPES OF ELECTRON MICROSCOPES; I. DIFFERENCES BETWEEN THE LIGHT AND ELECTRON MICROSCOPES; Part Two: METHODOLOGY; Chapter 5. INTRODUCTION; Chapter 6. HISTORICAL REVIEW; Chapter 7. OBTAINING THE SPECIMEN; A. PREPARATION OF EXCISED SPECIMENS; B. IN SITU FIXATION; C. PERFUSION; D. HANDLING OF SMALL SPECIMENS; E. HANDLING OF HUMAN MATERIAL; F. DISCUSSION; Chapter 8. FIXATION; A. OSMIUM TETROXIDE FIXATION; B. OTHER FIXATION MEDIA; C. DISCUSSION; Chapter 9. DEHYDRATION; A. DEHYDRATION MEDIA.
- B. DURATION OF DEHYDRATIONChapter 10. EMBEDDING; A. METHACRYLATE; B. WATER-SOLUBLE EMBEDDING MEDIA; C. POLYESTER RESINS; D. EPOXY RESINS; E. DISCUSSION; Chapter 11. MICROTOMY; A. PRINCIPLES OF MICROTOMY; B. ULTRAMICROTOMES; C. KNIVES; D. THE TROUGH; E. SECTION THICKNESS; F. DISCUSSION; Chapter 12. STAINING; A. PRINCIPLES OF STAINING; B. POSITIVE STAINING; C. NEGATIVE STAINING; Chapter 13. SPECIALIZED ELECTRON MICROSCOPE TECHNIQUES; A. ULTRASTRUCTURAL NUCLEOPROTEIN LOCALIZATION; B. ULTRASTRUCTURAL ENZYME CYTOCHEMISTRY; C. TRACERS IN ELECTRON MICROSCOPY.
- D. ULTRASTRUCTURAL IMMUNO-ELECTRON MICROSCOPYE. ELECTRON AUTORADIOGRAPHY; F. HIGH RESOLUTION ELECTRON MICROSCOPY; G. ELECTRON MICROSCOPIC ANALYSIS OF CELL FRACTIONS; H. CRYOFIXATION FOR ELECTRON MICROSCOPY; I. SURFACE EXAMINATION OF SMALL PARTICLES; J. QUANTITATIVE ELECTRON MICROSCOPY; APPENDICES; Appendix A:THE NATURE OF LIGHT; Appendix B: WAVELENGTH OF AN ELECTRON; Appendix C: SUMMATION AND RESOLUTION OF VECTORS; Appendix D: FUNDAMENTALS OF ELECTRONICS; Appendix E: DEPENDENCE OF CONDENSER APERTURE ANGLE ON FOCAL LENGTH; Appendix F: DEPTH OF FIELD; Appendix G: SPHERICAL ABERRATION.
- Appendix H: CHROMATIC ABERRATIONAppendix I: OPTICAL THEORY OF CYLINDRICAL LENSES; Appendix J: THEORY OF FRESNEL DIFFRACTION FRINGES; Appendix K: ELECTRON DIFFRACTION; Appendix L: SHADOW CASTING; LITERATURE CITED; REFERENCES; BIBLIOGRAPHY; GLOSSARY; AUTHOR INDEX; SUBJECT INDEX.