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Enzyme kinetics and mechanism. Part F, Detection and characterization of enzyme reaction intermediates /
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now wit...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego ; London :
Academic Press,
�2002.
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| Colección: | Methods in enzymology ;
v. 354. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- 1.Covalent Enzyme-Substrate Compounds: Detection and Catalytic Competence / Daniel L. Purich
- 2.Rapid Mix-Quench MALDI-TOF Mass Spectrometry for Analysis of Enzymatic Systems / Jeffrey W. Gross / Perry A. Frey
- 3.Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing Techniques / Donald J. Douglas / Lars Konermann
- 4.Trapping of [alpha]-Glycosidase Intermediates / Renee M. Mosi / Stephen G. Withers
- 5.Trapping Covalent Intermediates on [beta]-Glycosidases / Jacqueline Wicki / David R. Rose / Stephen G. Withers
- 6.2-Hydroxy-6-keto-nona-2,4-diene 1,9-Dioic Acid 5,6-Hydrolase: Evidence from [superscript 18]O Isotope Exchange for gem-Diol Intermediate / Timothy D. H. Bugg / Sarah M. Fleming / Thomas A. Robertson / G. John Langley
- 7.Nucleoside-Diphosphate Kinase: Structural and Kinetic Analysis of Reaction Pathway and Phosphohistidine Intermediate / Joel Janin / Dominique Deville-Bonne
- 8.Galactose-1-Phosphate Uridylyltransferase: Kinetics of Formation and Reaction of Uridylyl-Enzyme Intermediate in Wild-Type and Specifically Mutated Uridylyltransferases / Sandaruwan Geeganage / Perry A. Frey
- 9.Kinetic Evidence for Covalent Phosphoryl-Enzyme Intermediate in Phosphotransferase Activity of Human Red Cell Pyrimidine Nucleotidases / Adolfo Amici / Monica Emanuelli / Silverio Ruggieri / Nadia Raffaelli / Giulio Magni
- 10.Characterization of [alpha](2[approaches]6)-Sialyltransferase Reaction Intermediates: Use of Alternative Substrates to Unmask Kinetic Isotope Effects / Benjamin A. Horenstein / Michael Bruner
- 11.Use of Sodium Borohydride to Detect Acyl-Phosphate Linkages in Enzyme Reactions / Daniel L. Purich
- 12.Evidence for Phosphotransferases Phosphorylated on Aspartate Residue in N-Terminal DXDX(T/V) Motif / Jean-Francois Collet / Vincent Stroobant / Emile Van Schaftingen
- 13.MurC and MurD Synthetases of Peptidoglycan Biosynthesis: Borohydride Trapping of Acyl-Phosphate Intermediates / Ahmed Bouhss / Sebastien Dementin / Jean van Heijenoort / Claudine Parquet / Didier Blanot
- 14.Transaldolase B: Trapping of Schiff Base Intermediate between Dihydroxyacetone and [epsilon]-Amino Group of Active-Site Lysine Residue by Borohydride Reduction / Gunter Schneider / Georg A. Sprenger
- 15.T4 Endonuclease V: Use of NMR and Borohydride Trapping to Provide Evidence for Covalent Enzyme-Substrate Imine Intermediate / M. L. Dodson / Andrew J. Kurtz / R. Stephen Lloyd
- 16.Detection of Covalent Tetrahedral Adducts by Differential Isotope Shift [superscript 13]C NMR: Acetyl-Enzyme Reaction Intermediate Formed by 3-Hydroxy-3-methylglutaryl-CoA Synthase / Henry M. Miziorko / Dmitriy A. Vinarov
- 17.Detection of Intermediates in Reactions Catalyzed by PLP-Dependent Enzymes: O-Acetylserine Sulfhydrylase and Serine-Glyoxalate Aminotransferase / William E. Karsten / Paul F. Cook
- 18.Protein Tyrosine Phosphatases: X-Ray Crystallographic Observation of Cysteinyl-Phosphate Reaction Intermediate / David Barford
- 19.GTP:GTP Guanylyltransferase: Trapping Procedures for Detecting and Characterizing Chemical Nature of Enzyme-Nucleotide Phosphoramidate Reaction Intermediate / Jared L. Cartwright / Alexander G. McLennan
- 20.[gamma]-Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B / Holly G. Schnizer / Susan K. Boehlein / Jon D. Stewart / Nigel G. J. Richards / Sheldon M. Schuster
- 21.[gamma]-Glutamyltranspeptidase and [gamma]-Glutamyl Peptide Ligases: Fluorophosphonate and Phosphonodifluoromethyl Ketone Analogs as Probes of Tetrahedral Transition State and [gamma]-Glutamyl-Phosphate Intermediate / Jun Hiratake / Makoto Inoue / Kanzo Sakata
- 22.Stoichiometric Redox Titrations of Complex Metalloenzymes / Paul A. Lindahl
- 23.Urate Oxidase: Single-Turnover Stopped-Flow Techniques for Detecting Two Discrete Enzyme-Bound Intermediates / Peter A. Tipton
- 24.Nitric Oxide Synthase: Use of Stopped-Flow Spectroscopy and Rapid-Quench Methods in Single-Turnover Conditions to Examine Formation and Reactions of Heme-O[subscript 2] Intermediate in Early Catalysis / Chin-Chuan Wei / Zhi-Qiang Wang / Dennis J. Stuehr
- 25.Myeloperoxidase: Kinetic Evidence for Formation of Enzyme-Bound Chlorinating Intermediate / H. Brian Dunford / Leah A. Marquez-Curtis
- 26.Time-Resolved Resonance Raman Spectroscopy of Intermediates in Cytochrome Oxidase / Denis L. Rousseau / Sanghwa Han
- 27.Porphobilinogen Deaminase: Accumulation and Detection of Tetrapyrrole Intermediates Using Enzyme Immobilization / Alcira Batlle
- 28.Adenosylcobalamin-Dependent Glutamate Mutase: Pre-Steady-State Kinetic Methods for Investigating Reaction Mechanism / Hung-Wei Chih / Ipsita Roymoulik / Marja S. Huhta / Prashanti Madhavapeddi / E. Neil G. Marsh
- 29.Ribonucleotide Reductase: Kinetic Methods for Demonstrating Radical Transfer Pathway in Protein R2 of Mouse Enzyme in Generation of Tyrosyl Free Radical / Astrid Graslund
- 30.Galactose Oxidase: Probing Radical Mechanism with Ultrafast Radical Probe / Bruce P. Branchaud / B. Elizabeth Turner
- 31.Kinetic Characterization of Transient Free Radical Intermediates in Reaction of Lysine 2,3-Aminomutase by EPR Lineshape Analysis / Perry A. Frey / Christopher H. Chang / Marcus D. Ballinger / George H. Reed
- 32.Demonstration of Peroxodiferric Intermediate in M-Ferritin Ferroxidsase Reaction Using Rapid Freeze-Quench Mossbauer, Resonance Raman, and XAS Spectroscopies / Carsten Krebs / Dale E. Edmondson / Boi Hanh Huynh
- 33.A Survey of Covalent, Ionic, and Radical Intermediates in Enzyme-Catalyzed Reactions / R. Donald Allison / Daniel L. Purich.