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Digital microscopy /

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geo...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Sluder, Greenfield, Wolf, D. E.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Oxford : Academic, 2007.
Edición:3rd ed.
Colección:Methods in cell biology ; v. 81.
Temas:
Acceso en línea:Texto completo
Texto completo
Descripción
Sumario:The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods.
Descripción Física:1 online resource (xvii, 608 pages, 18 unnumbered pages of plates) : illustrations.
Bibliografía:Includes bibliographical references and index.
ISBN:0123740258
9780123740250
0080544347
9780080544342
1281025097
9781281025098
9786611025090
661102509X