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Methods in cell biology. Vol. XX /

METHODS IN CELL BIOLOGY,VOLUME 20.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Prescott, David M., 1926-2011
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1978.
Colección:Methods in cell biology.
Temas:
Acceso en línea:Texto completo

MARC

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245 0 0 |a Methods in cell biology.  |n Vol. XX /  |c edited by David M. Prescott. 
260 |a New York :  |b Academic Press,  |c 1978. 
300 |a 1 online resource (xix, 539 pages) :  |b illustrations. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in cell biology 
504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
505 0 |a Front Cover; Methods in Cell Biology, Volume XX; Copyright Page; Contents; List of Contributors; Preface; Chapter 1. Measuring Spontaneous Mutation Rates in Yeast; I. Introduction; II. Measuring the Spontaneous Mutation Rate in Mitotic Cells by Limiting a Required Nutrient; III. Measuring the Spontaneous Mutation Rate in Mitotic Cells Grown in Complete Medium; IV. Measuring the Spontaneous Mutation Rate during Meiosis; V. Conclusion; Appendix: Biometrical Aspects of Measuring Mutation Rates; References; Chapter 2. Manganese Mutagenesis in Yeast; I. Introduction 
505 8 |a II. Induction of Mitochondrial MutationsIII. Induction of Nuclear Mutations; IV. The Uptake of Manganese and Its General Effects on Yeast Cells; V. Other Mutagens Inducing Mutations Nonlethal for rho Factor; References; Chapter 3. Yeast Cell Selection and Synchrony: Density Gradients and Mating Factor Block; I. Introduction; II. lsopycnic Density Gradients; III. Isolation of Cells Labeled at Specific Periods of the Cell Cycle; IV. a- and a-Factor Selection and Synchronization of Haploid Yeast Cells; V. Discussion; References; Chapter 4. The Ribosomal Proteins of Saccharomyces cerevisiae 
505 8 |a I. Preparation of RibosomesII. Preparation of Ribosomal Proteins; III. Analysis of Ribosomal Proteins; IV. Conclusion; References; Note Added in Proof; Chapter 5. Isolation, Characterization, and Translation of mRNA from Yeast; I. Media, Cells, and Spheroplasts; II. Isolation of Polysomes, Single Ribosomes, and Ribosomal Subunits; III. Detection of mRNA in Polysomes and Subunits; IV. Preparation of RNA; V. Analysis of RNA on Polyacrylamide Gels; VI. Isolation of Poly-A-Containing RNA; VII. Analysis of 5'-Termini of mRNA; VIII. Translation of Yeast mRNA; References 
505 8 |a Chapter 6. Method for Yeast Protein Synthesis in a Cell-Free SysternI. Introduction; II. Early Yeast Cell-Free Systems; III. The S-30 Extract; IV. Assay of Protein Synthesis; V. Prospects; References; Chapeter 7. Preparation of Protoplasts of Schizosaccharomyces pombe; I. Introduction; II. Preparation of Protoplasts; III. Concluding Remarks; References; Chapter 8. Bulk Isolation of Yeast Mitochondria; I. Introduction; II. Materials and Methods; III.Breakage of Yeast Cells; IV.Isolation of Mitochondria; V.Properties of Isolated Mitochondria; VI.Discussion; References 
505 8 |a Chapter 9. Isolation, Regeneration, and Fusion of Protoplasts of PhycomycesI. Introduction; II. Material; III. Preparation of Cells; IV.Protoplast Isolation; V.Reversion of Protoplasts; VI.Protoplast Fusion; VII.Discussion; References; Chapter 10.The Neurospora Plasma Membrane: A New Experimental System for Investigating Eukaryote Surface Membrane Structure and Function; I. Introduction; II.Isolation of Neurospora Plasma Membranes; III.Properties of Isolated Neurospora Plasma Membranes; IV.Conclusion; References; Chapter 11. Staining Fungal Nuclei with Mithramycin; I.Introduction 
520 |a METHODS IN CELL BIOLOGY,VOLUME 20. 
546 |a English. 
650 0 |a Cytology  |x Technique. 
650 2 |a Cytological Techniques  |0 (DNLM)D003584 
650 6 |a Cytologie  |x Technique.  |0 (CaQQLa)201-0005040 
650 7 |a Cytology  |x Technique  |2 fast  |0 (OCoLC)fst00886306 
700 1 |a Prescott, David M.,  |d 1926-2011. 
776 0 8 |i Print version:  |t Methods in cell biology.  |d New York : Academic Press, 1978  |w (DLC) 64014220 
830 0 |a Methods in cell biology. 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9780125641203  |z Texto completo