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Subcellular components: preparation and fractionation
Subcellular Components: Preparation and Fractionation talks about cells and particles' components, including their preparation and fractionation. The book includes theories and answers to questions that are relevant to the study.
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London, Baltimore,
Butterworth; University Park Press,
1972.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Subcellular Components: Preparation and Fractionation; Copyright Page ; Table of Contents; CHAPTER 1. PRESSURE HOMOGENISATION OF MAMMALIAN CELLS; APPARATUS AND METHOD; RESULTS WITH SOLID TISSUES; RESULTS WITH SINGLE-CELL SUSPENSIONS; CONCLUSIONS; REFERENCES; CHAPTER 2. SOME METHODS FOR ISOLATING NUCLEI; CRITERIA FOR ASSESSING ISOLATION PROCEDURES; DETAILS OF SOME ISOLATION PROCEDURES; SUBFRACTIONATION OF NUCLEI; CONCLUSIONS; APPENDIX; REFERENCES; CHAPTER 3. THE BIOCHEMICAL PROPERTIES OF NUCLEI FRACTIONATED BY ZONAL CENTRIFUGATION; ZONAL CENTRIFUGATION; BIOCHEMICAL PROPERTIES.
- Nuclei of avian red cellsconclusions; references; chapter 4. preparation of mitochondria from animal tissues and yeasts; preparation of mitochondria: general principles; preparation of rat-liver mitochondria; preparation of rat-heart mitochondria; preparation of blowfly flight-muscle miochondria; preparation of yeast mitochondria; preparation of brain mitochondria; assessment of the preparation; references; chapter 5. preparation of lysosome-rich fractions with or without peroxisomes; discovery and nature of lysosomes and peroxisomes; centrifugal methods for hepatic lysosomes.
- Centrifugal methods for tissues other than livermethods besides centrifugation; conclusions and summary; references; chapter 6. purification of plasma-membrane fragments; liver and hepatoma plasma-membrane fragments; choice of methods for isolating plasma-membrane fragments from liver and hepatoma; details of selected procedures for the purification of plasma-membrane fragments from liver and hepatoma; separation of plasma-membrane fragments from other tissues; purity of plasma-membrane preparations; general comments; references.
- Chapter 7. the isolation of brush borders (microvilli) from the epithelial cells of mammalian intestinethe role of the brush border in transintestinal transport; methods available for the isolation of brush borders; the isolation of purified brush borders from rat-intestine epithelial cells; qualitative and quantitative criteria of the purity of isolated brush borders; references; chapter 8. preparation and properties of microsomal and submicrosomal fractions from secretory and non-secretory tissues; microsomal activities and functions; major procedures for microsomal preparations.
- FACTORS IMPORTANT IN ISOLATION PROCEDURESRECOMMENDED METHODS FOR THE ISOLATION OF MICROSOMES AND SUBMICROSOMAL FRACTIONS; COMPOSITION AND PROPERTIES OF MICROSOMAL AND SUBMICROSOMAL FRACTIONS; CONCLUDING REMARKS; REFERENCES; CHAPTER 9. ISOLATION OF ANIMAL POLYSOMES AND RIBOSOMES; ASPECTS OF POLYSOME STRUCTURE AND FUNCTION; METHODS OF ISOLATION; FRACTIONATION AND CHARACTERISATION OF POLYSOMES; ISOLATION OF POLYSOMES FROM SPECIFIC CELLS AND TISSUES; REFERENCES; ADDENDUM; References; CHAPTER 10. SEPARATION OF POLYSOMES, RIBOSOMES AND RIBOSOMAL SUBUNITS IN ZONAL ROTORS; EXPERIMENTAL PROCEDURES.