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Modern physical methods in biochemistry. Part B /
This volume completes the account of physical methods used in biochemistry that was covered in Volume 11A, which was published in 1985. It deals with fast reaction methods, high performance liquid chromatography of peptides and proteins, fluorescence spectroscopy, neutron and X-ray scattering techni...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Elsevier Science Pub. Co.,
1988.
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| Colección: | New comprehensive biochemistry ;
v. 11B. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Modern Physical Methods in Biochemistry: Part B; Preface; Contents; Chapter 1. Fluorescence Spectroscopy; Principles and Application to Biological Macromolecules; 1. The Phenomenon of Fluorescence; 2. Factors Aaffecting the Fluorescence Emission; 3. Time-Resolved Fluorescence Spectroscopy; 4. Harmonic-Content Frequency-Domain Fluorometry; 5. Summary; Acknowledgements; References; Chapter 2. Raman and Rresonance Raman Spectroscopy; 1. Introduction; 2. The Units Used in Raman Sspectroscopy; 3. A Model for Raman Scattering Based on Classical Physics
- 4. Raman and Resonance Raman Scattering: a Quantum Mechanical Interpretation5. Polarisation Properties of Raman Scattering; 6. Basic Experimental Aspects; 7. Raman Studies on Biological Materials; References; Chapter 3. Rapid Reaction Methods in Biochemistry; 1. Introduction; 2. Continuous flow; 3. Stopped flow; 4. Combinations of Flash Photolysis with Other Techniques; 5. Temperature Jump; 6. Miscellaneous Methods; 7. Data Reduction; References; Chapter 4. High Performance Liquid Chromatography of Nucleic Acids; 1. Introduction; 2. Techniques; 3. Applications; 4. Concluding remarks
- AcknowledgementsReferences; Chapter 5. Reversed Phase High Performance Liquid Chromatography of Peptides and Proteins; 1. Introduction; 2. Retention Relationships of Peptides in RP-HPLC; 3. The Relationship Between Peptide Retention Behaviour and Hydrophobicity Coefficients; 4. Bandwidth Relationships of Peptides in RP-HPLC; 5. Dynamic Models for Interconverting Systems; 6. Conclusion; Acknowledgements; References; Chapter 6. X-ray and Neutron Solution Scattering; 1. Introduction; Part A: Theoretical and Practical Aspects; 2. Theory of X-ray and Eutron Scattering
- 3. Experimental Practice and InstrumentationPart B: Biochemical Applications to Proteins, Carbohydrates, Lipids and Nucleic Acids; 4. Applications of X-ray and Neutron Scattering; 5. Conclusions; Acknowledgements; References; Chapter 7. Electron Microscopy; 1. Introduction; 2. Negative Staining and Metal Shadowing; 3. Thin Sectioning; 4. Low-Temperature Techniques; 5. Conclusions; Acknowledgements; References; Subject Index