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Laboratory techniques in biochemistry and molecular biology. Volume 13, Monoclonal antibody technology: the production and characterization of rodent and human hybridomas /
This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Oxford :
North-Holland Pub. Co.,
1984.
|
| Colección: | Laboratory techniques in biochemistry and molecular biology ;
13. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 082 | 0 | 4 | |a 572.072/1 |2 23 |
| 100 | 1 | |a Campbell, Ailsa M. | |
| 245 | 1 | 0 | |a Laboratory techniques in biochemistry and molecular biology. |n Volume 13, |p Monoclonal antibody technology: the production and characterization of rodent and human hybridomas / |c Ailsa M. Campbell. |
| 260 | |a Amsterdam ; |a Oxford : |b North-Holland Pub. Co., |c 1984. | ||
| 300 | |a 1 online resource (xiv, 265 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology ; |v 13 | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Monoclonal Antibody Technology; Copyright Page; Contents; Preface; Acknowledgements; Abbreviations; Chapter 1. General properties and applications of monoclonal antibodies; 1.1. Introduction; 1.2. Comparison of monoclonal antibodies and conventional antiserum; 1.3. Application of monoclonal antibodies; Chapter 2. Assay techniques; 2.1. General assay requirements; 2.2. Theoretical considerations; 2.3. Practical considerations; 2.4. Antibody sampling; 2.5. Types of assay; 2.6. Solid-phase-assays; 2.7. Soluble-phase systems; 2.8. Cellular assays; 2.9. Biological assays | |
| 505 | 8 | |a 2.10 Immunocytochemical assaysChapter 3. Selection of animals and cell lines; 3.1. Choice of animal; 3.2. Choice of cell lines; 3.3. Selective drug markers; 3.4. The mouse system; 3.5. The rat system; 3.6. The human system; 3.7. T cell lines; Chapter 4. Immunisation; 4.1. Introduction; 4.2. Immunisation schedules; 4.3. Immunisation in vitro; 4.4. Isotype enrichment; Chapter 5. Cell culture requirements for hybridomas; 5.1. Introduction; 5.2. Basic requirements; 5.3. Basic cell-culture techniques; 5.4. Contamination; 5.5. Feeder cells; Chapter 6. Fusion procedures | |
| 505 | 8 | |a 6.1. The use of polyethylene glycol6.2. The components of HAT medium; 6.3. Selection of hybridomas by fluorescence activated cell sorting; 6.4. Preparation of stock solutions; 6.5. Fusion frequencies and plating densities; 6.6. Fusion protocols for mouse experiments; Chupter 7. Transformation; 7.1. Introduction; 7.2. Pre-selection of lymphocytes of pre-defined specificity; 7.3. Pre-selection of T lymphocytes; 7.4. Transformation; 7.5. Transformation followed by fusion; Chapter 8. Selection and cloning; 8.1. Early feeding and assay of fusions; 8.2. Failure of fusions | |
| 505 | 8 | |a 8.3. Cloning of hybridomas8.4. Failure of cloning; 8.5. Continuation of cloning; Chapter 9. Antibody production and purification; 9.1. Maintenance of cell stocks; 9.2. Expansion of hybridomas in vitro; 9.3. Expansion of hybridomas in vivo; 9.4. Failure in expansion; 9.5. Storage of antibody; 9.6. Concentrating the antibody; 9.7. Purification of the antibody; Chapter 10. Characterisation of monoclonal antibodies; 10.1. Introduction; 10.2. Determination of antibody class; 10.3. Proof that the antibody is monoclonal; 10.4. Epitope analysis; 10.5. Determination of antibody affinity | |
| 505 | 8 | |a 10.6. Karyotype analysis of hybridomasAppendix 1. Animal handling techniques; Appendix 2. Addresses of suppliers and manufacturers; Appendix 3. Protocols for polyacrylamide gel electrophoresis (PAGE); References; Addendum; Subject Index | |
| 546 | |a English. | ||
| 650 | 0 | |a Biochemistry |x Technique. | |
| 650 | 0 | |a Molecular biology |x Technique. | |
| 650 | 6 | |a Biochimie |x Technique. |0 (CaQQLa)201-0003852 | |
| 650 | 6 | |a Biologie mol�eculaire |x Technique. |0 (CaQQLa)201-0196285 | |
| 650 | 7 | |a Biochemistry |x Technique. |2 fast |0 (OCoLC)fst00831987 | |
| 650 | 7 | |a Molecular biology |x Technique. |2 fast |0 (OCoLC)fst01024758 | |
| 776 | |z 0-444-80592-3 | ||
| 830 | 0 | |a Laboratory techniques in biochemistry and molecular biology ; |v 13. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780444805928 |z Texto completo |