Laboratory techniques in biochemistry and molecular biology. Volume 18, Methods of cell separation /

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specif...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Sharpe, Paul T.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Oxford : North-Holland Pub. Co., 1988.
Colección:Laboratory techniques in biochemistry and molecular biology ; 18.
Temas:
Cell separation.
Molecular biology > Technique.
Cell Separation
Cellules > S�eparation.
Biologie mol�eculaire > Technique.
Cell separation
Molecular biology > Technique
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Methods of Cell Separation; Copyright Page; Contents; Abbreviations; Chapter 1. Introduction; Chapter 2. Methods of cell counting and assaying cell viability; 2.1. Direct counting methods; 2.2. Indirect cell counting methods; 2.3. Cell viability; Chapter 3. Centrifugation; 3.1. Introduction; 3.2. Differential pelleting; 3.3. Density gradient centrifugation; 3.4. Density gradient cell separation; Chapter 4. Unit gravity sedimentation; 4.1. Introduction; 4.2. STAPUT apparatus; 4.3. Other types of apparatus; Chapter 5. Centrifugal elutriation; 5.1. Introduction
  • 5.2. Beckman JE-6B elutriator system5.3. Examples of cell separations; 5.4. Separation of cells with different densities; Chapter 6. Aqueous two-phase partition; 6.1. Introduction; 6.2. Factors that influence partition; 6.3. Cell partition; 6.4. Single-step partition; 6.5. Two-step partition; 6.6. Multiple partition-thin layer countercurrent distribution; 6.7. Fractionation of human peripheral blood lymphocytes with TLCCD; 6.8. Separation of differentiating D. discoideum cells; 6.9. Effect of protease treatment on cell partition; 6.10. Computer analysis of TLCCD profiles
  • Chapter 7. Electrophoresis7.1. Introduction; 7.2. Free-flow electrophoresis; 7.3. Biostream; 7.4. Density gradient electrophoresis; Specific surface methods
  • an introduction to Chapters 8-13; Chapter 8. Affinity chromatography; 8.1. lntroduction; 8.2. Types of ligand interaction; 8.3. Types of adsorbant; 8.4. Sepharose 6MB macracrobeads; 8.5. Affi-gel; 8.6. lmmobilisation of ligands through cleavable bonds; 8.7. Glass and plastic beads; Chapter 9. Affinity separation on fibres and surfaces; 9.1. Introduction; 9.2. Separation on unmodified fibres; 9.3. Separation on modified fibres
  • 9.4. Separation of unmodified surfaces9.5. Separation on ligand coated surfaces; 9.6. Separation on sephadex G-10; Chapter 10. Affinity partition; 10.1. Introduction; 10.2. Antibody affinity cell partition; Chapter 11. Flow sorting; 11.1. Introduction; 11.2. Principle; 11.3. Signals produced; 1.4. Cell separation; Chapter 12. Magnetic cell sorting; 12.1. lntroduction; 12.2. Types of apparatus; 12.3. Preparation of magnetic microspheres; Chapter 13. Separation of cells in different phases of the cell cycle; 13.1. Introduction; 13.2. Centrifugation; 13.3. Centrifugal elutriation
  • 13.4. Unit gravity sedimentation13.5. Phase partition; 13.6. Flow sorting; 13.7. Mitotic wash-off; Appendix; References; General references for further reading; Subject index

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