Cargando…
Laboratory techniques in biochemistry and molecular biology. Volume 18, Methods of cell separation /
Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specif...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Oxford :
North-Holland Pub. Co.,
1988.
|
| Colección: | Laboratory techniques in biochemistry and molecular biology ;
18. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000Ma 4500 | ||
|---|---|---|---|
| 001 | SCIDIR_ocn646756791 | ||
| 003 | OCoLC | ||
| 005 | 20231117033230.0 | ||
| 006 | m o d | ||
| 007 | cr cn||||||||| | ||
| 008 | 850930s1988 ne a o 000 0 eng d | ||
| 040 | |a E7B |b eng |e pn |c E7B |d OCLCQ |d OPELS |d OCLCQ |d OCLCF |d OCLCQ |d DEBSZ |d LEAUB |d VLY |d S2H |d OCLCO |d OCLCQ |d OCLCO | ||
| 019 | |a 1162202120 | ||
| 020 | |a 0444809279 |q (electronic bk.) | ||
| 020 | |a 9780444809278 |q (electronic bk.) | ||
| 020 | |a 9786611716264 | ||
| 020 | |a 6611716262 | ||
| 020 | |a 0080858872 | ||
| 020 | |a 9780080858876 | ||
| 035 | |a (OCoLC)646756791 |z (OCoLC)1162202120 | ||
| 050 | 4 | |a QH585.5.C44 |b L3318 1988eb | |
| 082 | 0 | 4 | |a 572 |2 23 |
| 100 | 1 | |a Sharpe, Paul T. | |
| 245 | 1 | 0 | |a Laboratory techniques in biochemistry and molecular biology. |n Volume 18, |p Methods of cell separation / |c Paul T. Sharpe. |
| 260 | |a Amsterdam ; |a Oxford : |b North-Holland Pub. Co., |c 1988. | ||
| 300 | |a 1 online resource (xiii, 272 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology ; |v 18 | |
| 520 | |a Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Methods of Cell Separation; Copyright Page; Contents; Abbreviations; Chapter 1. Introduction; Chapter 2. Methods of cell counting and assaying cell viability; 2.1. Direct counting methods; 2.2. Indirect cell counting methods; 2.3. Cell viability; Chapter 3. Centrifugation; 3.1. Introduction; 3.2. Differential pelleting; 3.3. Density gradient centrifugation; 3.4. Density gradient cell separation; Chapter 4. Unit gravity sedimentation; 4.1. Introduction; 4.2. STAPUT apparatus; 4.3. Other types of apparatus; Chapter 5. Centrifugal elutriation; 5.1. Introduction | |
| 505 | 8 | |a 5.2. Beckman JE-6B elutriator system5.3. Examples of cell separations; 5.4. Separation of cells with different densities; Chapter 6. Aqueous two-phase partition; 6.1. Introduction; 6.2. Factors that influence partition; 6.3. Cell partition; 6.4. Single-step partition; 6.5. Two-step partition; 6.6. Multiple partition-thin layer countercurrent distribution; 6.7. Fractionation of human peripheral blood lymphocytes with TLCCD; 6.8. Separation of differentiating D. discoideum cells; 6.9. Effect of protease treatment on cell partition; 6.10. Computer analysis of TLCCD profiles | |
| 505 | 8 | |a Chapter 7. Electrophoresis7.1. Introduction; 7.2. Free-flow electrophoresis; 7.3. Biostream; 7.4. Density gradient electrophoresis; Specific surface methods -- an introduction to Chapters 8-13; Chapter 8. Affinity chromatography; 8.1. lntroduction; 8.2. Types of ligand interaction; 8.3. Types of adsorbant; 8.4. Sepharose 6MB macracrobeads; 8.5. Affi-gel; 8.6. lmmobilisation of ligands through cleavable bonds; 8.7. Glass and plastic beads; Chapter 9. Affinity separation on fibres and surfaces; 9.1. Introduction; 9.2. Separation on unmodified fibres; 9.3. Separation on modified fibres | |
| 505 | 8 | |a 9.4. Separation of unmodified surfaces9.5. Separation on ligand coated surfaces; 9.6. Separation on sephadex G-10; Chapter 10. Affinity partition; 10.1. Introduction; 10.2. Antibody affinity cell partition; Chapter 11. Flow sorting; 11.1. Introduction; 11.2. Principle; 11.3. Signals produced; 1.4. Cell separation; Chapter 12. Magnetic cell sorting; 12.1. lntroduction; 12.2. Types of apparatus; 12.3. Preparation of magnetic microspheres; Chapter 13. Separation of cells in different phases of the cell cycle; 13.1. Introduction; 13.2. Centrifugation; 13.3. Centrifugal elutriation | |
| 505 | 8 | |a 13.4. Unit gravity sedimentation13.5. Phase partition; 13.6. Flow sorting; 13.7. Mitotic wash-off; Appendix; References; General references for further reading; Subject index | |
| 546 | |a English. | ||
| 650 | 0 | |a Cell separation. | |
| 650 | 0 | |a Molecular biology |x Technique. | |
| 650 | 2 | |a Cell Separation |0 (DNLM)D002469 | |
| 650 | 6 | |a Cellules |x S�eparation. |0 (CaQQLa)201-0009729 | |
| 650 | 6 | |a Biologie mol�eculaire |x Technique. |0 (CaQQLa)201-0196285 | |
| 650 | 7 | |a Cell separation |2 fast |0 (OCoLC)fst00850234 | |
| 650 | 7 | |a Molecular biology |x Technique |2 fast |0 (OCoLC)fst01024758 | |
| 776 | |z 0-444-80930-9 | ||
| 776 | |z 0-444-80927-9 | ||
| 830 | 0 | |a Laboratory techniques in biochemistry and molecular biology ; |v 18. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780444809278 |z Texto completo |