Laboratory techniques in biochemistry and molecular biology. Volume 14, A guidebook to lipoprotein technique /

This volume is a comprehensive collection of methods for the isolation, characterization, analysis and estimation of soluble lipoproteins. It surveys each aspect of lipoprotein technology in a critical manner which will enable the investigator to select the methods most relevant to his requirements....

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Mills, G. L.
Otros Autores: Lane, Patricia A., Weech, P. K.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Oxford : North-Holland Pub. Co., 1984.
Colección:Laboratory techniques in biochemistry and molecular biology ; 14.
Temas:
Biochemistry > Technique.
Molecular biology > Technique.
Biochimie > Technique.
Biologie mol�eculaire > Technique.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; A Guidebook to Lipoprotein Technique; Copyright Page; Contents; Preface; Glossary of lipoprotein nomenclature as used in this manual; Glossary of apo-lipoprotein nomenclature as used in this manual; Chapter 1. Brief introduction to the plasma lipoproteins; 1.1. Nomenclature of the lipoproteins; 1.2. Nomenclature of apo-lipoproteins; Chapter 2. The isolation and purification of plasma lipoproteins; 2.1. Isolation by ultracentrifugation; 2.2. Isolation by precipitation; 2.3. Isolation by chromatography; 2.4. Isolation by preparative electrophoresis
  • 2.5. The isolation of Lp(a) and LP-X2.6. The preservation of isolated lipoproteins; Chapter 3. The characterisation of plasma lipoproteins: intro: duction to principles and methods; 3.1. The purity of lipoprotein preparations; 3.2. Physical and chemical characterisation; 3.3. Heterogeneity and polydispersity; Chapter 4. Physical characterisation of lipoproteins; 4.1. The analytical ultracentrifuge; 4.2. Partial specific volume (hydrated density); 4.3. The analysis of polydispersity in the preparative ultracentrifuge; 4.4. Gel filtration; 4.5. Electron microscopy
  • Chapter 5. Electrophoretic analysis of intact lipoproteins5.1. Preparation of samples; 5.2. Electrophoresis on paper; 5.3. Electrophoresis on cellulose acetate membranes; 5.4. Electrophoresis in agarose gel; 5.5. Electrophoresis in polyacrylamide gel; 5.6. Immunoelectrophoresis; 5.7. Two-dimensial immuno-electrophoresis; 5.8. Isoelectric focussing; Chapter 6. The chemical characterisation of lipoproteins; 6.1. Estimation of total lipoprotein; 6.2. Separation of the lipid and protein moieties; 6.3. Analysis of the lipid moiety; 6.4. Analysis of the protein moiety
  • 6.5. Qualitative analysis of apo-lipoproteins by immunodiffusion6.6. Identification and estimation of carbohydrates; 6.7. Quantitative analysis of lipoproteins by micro-scale thin-layer chromatography; Chapter 7. The estimation of lipoproteins; 7.1. Estimation of lipoproteins by refractometry; 7.2. Nephelometry; 7.3. The estimation of lipoproteins by chemical methods; 7.4. Estimation of LP-X; 7.5. Immunoassay of lipoproteins; Chapter 8. Isolation and assay of apo-lipoproteins; 8.1. Isolation of apo-lipoprotein A-1; 8.2. Isolation of apo-lipoprotein A-II; 8.3. Isolation of apo-lipoprotein A-IV
  • 8.4. Isolation of apo-lipoprotein B8.5. Isolation of the C apo-lipoproteins; 8.6. Isolation of apo-lipoprotein D; 8.7. Isolation of apo-lipoprotein E; 8.8. Isolation of apo-lipoprotein F; 8.9. The estimation of apo-lipoproteins; Appendix 1. The collection and preservation of blood plasma; Appendix 2. Methods of concentrating lipoprotein solutions; Appendix 3. The extraction of lipid from lipoproteins; Appendix 4. The preparation of stains

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