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Laboratory techniques in biochemistry and molecular biology. Volume 14, A guidebook to lipoprotein technique /
This volume is a comprehensive collection of methods for the isolation, characterization, analysis and estimation of soluble lipoproteins. It surveys each aspect of lipoprotein technology in a critical manner which will enable the investigator to select the methods most relevant to his requirements....
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Oxford :
North-Holland Pub. Co.,
1984.
|
| Colección: | Laboratory techniques in biochemistry and molecular biology ;
14. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 082 | 0 | 4 | |a 572/.36 |2 23 |
| 100 | 1 | |a Mills, G. L. | |
| 245 | 1 | 0 | |a Laboratory techniques in biochemistry and molecular biology. |n Volume 14, |p A guidebook to lipoprotein technique / |c G.L. Mills and Patricia A. Lane and P.K. Weech. |
| 246 | 3 | 0 | |a Guidebook to lipoprotein technique |
| 260 | |a Amsterdam ; |a Oxford : |b North-Holland Pub. Co., |c 1984. | ||
| 300 | |a 1 online resource (xiv, 512 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Laboratory techniques in biochemistry and molecular biology ; |v 14 | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a This volume is a comprehensive collection of methods for the isolation, characterization, analysis and estimation of soluble lipoproteins. It surveys each aspect of lipoprotein technology in a critical manner which will enable the investigator to select the methods most relevant to his requirements. Having made his choice, he will then find a detailed description of how to perform the technique. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; A Guidebook to Lipoprotein Technique; Copyright Page; Contents; Preface; Glossary of lipoprotein nomenclature as used in this manual; Glossary of apo-lipoprotein nomenclature as used in this manual; Chapter 1. Brief introduction to the plasma lipoproteins; 1.1. Nomenclature of the lipoproteins; 1.2. Nomenclature of apo-lipoproteins; Chapter 2. The isolation and purification of plasma lipoproteins; 2.1. Isolation by ultracentrifugation; 2.2. Isolation by precipitation; 2.3. Isolation by chromatography; 2.4. Isolation by preparative electrophoresis | |
| 505 | 8 | |a 2.5. The isolation of Lp(a) and LP-X2.6. The preservation of isolated lipoproteins; Chapter 3. The characterisation of plasma lipoproteins: intro: duction to principles and methods; 3.1. The purity of lipoprotein preparations; 3.2. Physical and chemical characterisation; 3.3. Heterogeneity and polydispersity; Chapter 4. Physical characterisation of lipoproteins; 4.1. The analytical ultracentrifuge; 4.2. Partial specific volume (hydrated density); 4.3. The analysis of polydispersity in the preparative ultracentrifuge; 4.4. Gel filtration; 4.5. Electron microscopy | |
| 505 | 8 | |a Chapter 5. Electrophoretic analysis of intact lipoproteins5.1. Preparation of samples; 5.2. Electrophoresis on paper; 5.3. Electrophoresis on cellulose acetate membranes; 5.4. Electrophoresis in agarose gel; 5.5. Electrophoresis in polyacrylamide gel; 5.6. Immunoelectrophoresis; 5.7. Two-dimensial immuno-electrophoresis; 5.8. Isoelectric focussing; Chapter 6. The chemical characterisation of lipoproteins; 6.1. Estimation of total lipoprotein; 6.2. Separation of the lipid and protein moieties; 6.3. Analysis of the lipid moiety; 6.4. Analysis of the protein moiety | |
| 505 | 8 | |a 6.5. Qualitative analysis of apo-lipoproteins by immunodiffusion6.6. Identification and estimation of carbohydrates; 6.7. Quantitative analysis of lipoproteins by micro-scale thin-layer chromatography; Chapter 7. The estimation of lipoproteins; 7.1. Estimation of lipoproteins by refractometry; 7.2. Nephelometry; 7.3. The estimation of lipoproteins by chemical methods; 7.4. Estimation of LP-X; 7.5. Immunoassay of lipoproteins; Chapter 8. Isolation and assay of apo-lipoproteins; 8.1. Isolation of apo-lipoprotein A-1; 8.2. Isolation of apo-lipoprotein A-II; 8.3. Isolation of apo-lipoprotein A-IV | |
| 505 | 8 | |a 8.4. Isolation of apo-lipoprotein B8.5. Isolation of the C apo-lipoproteins; 8.6. Isolation of apo-lipoprotein D; 8.7. Isolation of apo-lipoprotein E; 8.8. Isolation of apo-lipoprotein F; 8.9. The estimation of apo-lipoproteins; Appendix 1. The collection and preservation of blood plasma; Appendix 2. Methods of concentrating lipoprotein solutions; Appendix 3. The extraction of lipid from lipoproteins; Appendix 4. The preparation of stains | |
| 546 | |a English. | ||
| 650 | 0 | |a Biochemistry |x Technique. | |
| 650 | 0 | |a Molecular biology |x Technique. | |
| 650 | 6 | |a Biochimie |x Technique. |0 (CaQQLa)201-0003852 | |
| 650 | 6 | |a Biologie mol�eculaire |x Technique. |0 (CaQQLa)201-0196285 | |
| 650 | 7 | |a Biochemistry |x Technique. |2 fast |0 (OCoLC)fst00831987 | |
| 650 | 7 | |a Molecular biology |x Technique. |2 fast |0 (OCoLC)fst01024758 | |
| 700 | 1 | |a Lane, Patricia A. | |
| 700 | 1 | |a Weech, P. K. | |
| 776 | |z 0-444-80591-5 | ||
| 830 | 0 | |a Laboratory techniques in biochemistry and molecular biology ; |v 14. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780444805911 |z Texto completo |