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Methods in cell biology. Volume 7 /

METHODS IN CELL BIOLOGY,VOLUME 7.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Prescott, David M. (David Martin), 1948-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1973.
Colección:Methods in cell biology ; v. 7.
Temas:
Acceso en línea:Texto completo

MARC

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245 0 0 |a Methods in cell biology.  |n Volume 7 /  |c edited by David M. Prescott. 
260 |a New York :  |b Academic Press,  |c 1973. 
300 |a 1 online resource (xiii, 492 pages) :  |b illustrations. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in cell biology ;  |v 7 
504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
505 0 |a Cover; Contributors to This Volume; Contents; List of Contributors; Preface; Contents of Previous Volumes; Chapter 1. The Isolation of RNA from Mammalian Cells; I. Introduction; II. The Isolation of Subcellular Components; III. The Extraction of RNA; IV. RNA Purification Procedures; V. The Isolation of Messenger RNA; References; Chapter 2. Preparation of RNA from Animal Cells; I. Introduction; II. Labeling of RNA with Radioactive Precursors; III. Preparation of Rapidly Labeled Nuclear RNA; IV. Preparation of Ribosomal RNA; V. Preparation of Messenger RNA; VI. Preparation of Transfer RNA 
505 8 |a VII. Preparation of Mitochondrial RNAVIII. Concluding Remarks; References; Chapter 3. Detection and Utilization of Poly(A) Sequennces in Messenger RNA; I. Introduction; II. Detection and Characterization; References; Chapter 4. Recent Advances in the Preparation of Mammalian Ribosomes and Analysis of Their Protein Composition; I. Introduction; II. Preparation of Ribosomes; III. Preparation of Ribosomal Subunits; IV. Preparation of Total Protein from Ribosomes and Ribosomal Subunits; V. Analysis of Ribosomal Proteins; VI. Molecular Weight Distribution and Number of Ribosomal Proteins 
505 8 |a VII. Purified ProteinsVIII. Heterogeneity of Ribosomal Proteins; IX. Methods for Correlating Structure and Function; X. Membrane-Bound Ribosomes; XI. Phosphorylation of Ribosomal Proteins; XII. Translation of Specific Species of Messenger RNA into Proteins of Proven Structure; XIII. Concluding Remarks; References; Chapter 5. Methods for the Extraction and Purification of Deoxyribonucleic Acids from Eukaryote Cells; I. Introduction; II. Physical-Chemical Characteristics of DNA; III. General Methodology; IV. Concluding Remarks; V. Glossary of Reagents; References 
505 8 |a Chapter 6. Electron Microscopic Visualization of DNA in Association with Cellular ComponentsI. Introduction; II. Isolation of DNA-Protein Complexes; III. Electron Microscopy; IV. Direct Visualization of Defined Complexes; V. Phage-Membrane Association: A Combination of Two Methods; VI. Eukaryote Chromatin Fibers; References; Chapter 7. Autoradiography of Individual DNA Molecules; I. Introduction; II. Labeling of DNA; III. Spreading of DNA; IV. Autoradiography of DNA; V. Examples of Autoradiographs; References; Chapter 8. HeLa Cell Plasma Membranes; I. Introduction 
505 8 |a II. Preparation of HeLa Cell Plasma MembranesIII. Uses of the Method; IV. Discussion; References; Addendum; Chapter 9. Mass Enucleation of Cultured Animal Cells; I. Introduction; II. Cells; III. Manufacture of Plastic Coverslips; IV. Enucleation; V. Viability of Enucleated Cells; VI. Virus Activities in Enucleated Cells; VII. Viability of Nuclei Removed by Centrifugation; References; Chapter 10. The Production of Mass Populations of Anucleate Cytoplasms; I. Introduction; II. Preparation of Lucite Plates; III. Plating of Cells; IV. Enucleation 
520 |a METHODS IN CELL BIOLOGY,VOLUME 7. 
546 |a English. 
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650 0 |a Biology  |x Technique. 
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650 6 |a Biologie  |x Technique.  |0 (CaQQLa)201-0020329 
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650 7 |a Cytology  |x Technique  |2 fast  |0 (OCoLC)fst00886306 
650 7 |a Biologia celular.  |2 larpcal 
700 1 |a Prescott, David M.  |q (David Martin),  |d 1948- 
776 0 8 |i Print version:  |t Methods in cell biology. Volume VII.  |d New York : Academic Press, 1973  |w (DLC) 74641206 
830 0 |a Methods in cell biology ;  |v v. 7. 
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