Methods in cell biology. Volume 8 /

METHODS IN CELL BIOLOGY,VOLUME 8.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Prescott, David M. (David Martin), 1948-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1974.
Colección:Methods in cell biology ; v. 8.
Temas:
Cytology > Technique.
Biology > Technique.
Cytological Techniques
Cytologie > Technique.
Biologie > Technique.
SCIENCE > Life Sciences > Cell Biology.
Biology > Technique
Cytology > Technique
Biologia celular.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover; Contributors to This Volume; Contents; List of Contributors; Preface; Contents of Previous Volumes; Chapter 1. Methods for Selecting and Studying Temperature-Sensitive Mutants of BHK-21 Cells; I. Introduction; II. Choice of Cell Lines and Temperature; III. Mutagenesis; IV. Selection; V. Isolation of Surviving Cells and Testing for ts Phenotype; VI. Frequency and General Behavior of the ts BHK Mutants; VII. Complementation Tests and Methods to Test Dominance or Recessiveness of the ts Mutations; VIII. Nature of the ts Mutants; IX. Conclusions; References
  • Chapter 2. Induction and Isolation of Auxotrophic Mutants in Mammalian CellsI. Introduction; II. Procedures; III. Special Techniques, Equipment, and Reagents; IV. General Discussion; References; Chapter 3. Isolation of Temperature-Sensitive Mutants of Mammalian Cells; I. Introduction; II. Theoretical Considerations; III. Methods; IV. Discussion; References; Chapter 4. Preparation and Use of Replicate Mammalian Cell Cultures; I. Introduction; II. Replicate Culture Planting Device; III. Preparation and Use of Replicate Mammalian Cell Cultures; IV. Problems, and Some Suggestions
  • V. Quantitation of ResultsAppendix: Standardization of Hemacytometer and Coulter Counting Methods; References; Chapter 5. Methods for Obtaining Revertants of Transformed cells; I. Introduction; II. Isolation of Revertants from Transformed Cell Lines; III. Properties of Revertant Cells; IV. Conclusion; References; Chapter 6. Monolayer Mass Culture on Disposable Plastic Spirals; I. Introduction; II. Apparatus; III. Method; IV. Harvesting; V. Uses; References; Chapter 7. A Method for Cloning Anchorage-Dependent Cells in Agarose; I. Introduction; II. Procedure; III. Discussion; References
  • Chapter 8. Repetitive Synchronization of Human Lymphoblast Cultures with Excess ThymidineI. Introduction; II. Methods; III. Characterization of the Synchronization; IV. Concluding Remarks; References; Chapter 9. Uses of Enucleated Cells; I. Introduction; II. The Technology of Enucleation; III. Uses and Properties of Enucleated Cells; IV. Summary; References; Chapter 10. Enucleation of Somatic Cells with Cytochalasin B; I. Introduction; II. Enucleation of Somatic Cells Attached to the Inner Surface of Ultracentrifuge Nitrocellulose Tubes; III. Enucleation of Somatic Cells on Plastic Coverslips
  • IV. Results and DiscussionReferences; Chapter 11. Isolation of Mammalian Heterochromatin and Euchromatin; I. Introduction; II. Isolation of Nuclei; III. Isolation of Heterochromatin and Euchromatin; IV. Conclusion; References; Chapter 12. Measurements of Mammalian Cellular DNA and Its Localization in Chromosomes; I. Introduction; II. Banding Techniques; III. Flow Microfluorometric Analysis; IV. Applications of Methods to Cells in Culture; V. Summary; References; Chapter 13. Large-Scale Isolation of Nuclear Membranes from Bovine Liver; I. Introduction

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