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Methods in cell biology. Volume 8 /

METHODS IN CELL BIOLOGY,VOLUME 8.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Prescott, David M. (David Martin), 1948-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1974.
Colección:Methods in cell biology ; v. 8.
Temas:
Acceso en línea:Texto completo

MARC

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245 0 0 |a Methods in cell biology.  |n Volume 8 /  |c edited by David M. Prescott. 
260 |a New York :  |b Academic Press,  |c 1974. 
300 |a 1 online resource (xiii, 464 pages) :  |b illustrations. 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in cell biology ;  |v v. 8 
504 |a Includes bibliographical references and index. 
588 0 |a Print version record. 
505 0 |a Cover; Contributors to This Volume; Contents; List of Contributors; Preface; Contents of Previous Volumes; Chapter 1. Methods for Selecting and Studying Temperature-Sensitive Mutants of BHK-21 Cells; I. Introduction; II. Choice of Cell Lines and Temperature; III. Mutagenesis; IV. Selection; V. Isolation of Surviving Cells and Testing for ts Phenotype; VI. Frequency and General Behavior of the ts BHK Mutants; VII. Complementation Tests and Methods to Test Dominance or Recessiveness of the ts Mutations; VIII. Nature of the ts Mutants; IX. Conclusions; References 
505 8 |a Chapter 2. Induction and Isolation of Auxotrophic Mutants in Mammalian CellsI. Introduction; II. Procedures; III. Special Techniques, Equipment, and Reagents; IV. General Discussion; References; Chapter 3. Isolation of Temperature-Sensitive Mutants of Mammalian Cells; I. Introduction; II. Theoretical Considerations; III. Methods; IV. Discussion; References; Chapter 4. Preparation and Use of Replicate Mammalian Cell Cultures; I. Introduction; II. Replicate Culture Planting Device; III. Preparation and Use of Replicate Mammalian Cell Cultures; IV. Problems, and Some Suggestions 
505 8 |a V. Quantitation of ResultsAppendix: Standardization of Hemacytometer and Coulter Counting Methods; References; Chapter 5. Methods for Obtaining Revertants of Transformed cells; I. Introduction; II. Isolation of Revertants from Transformed Cell Lines; III. Properties of Revertant Cells; IV. Conclusion; References; Chapter 6. Monolayer Mass Culture on Disposable Plastic Spirals; I. Introduction; II. Apparatus; III. Method; IV. Harvesting; V. Uses; References; Chapter 7. A Method for Cloning Anchorage-Dependent Cells in Agarose; I. Introduction; II. Procedure; III. Discussion; References 
505 8 |a Chapter 8. Repetitive Synchronization of Human Lymphoblast Cultures with Excess ThymidineI. Introduction; II. Methods; III. Characterization of the Synchronization; IV. Concluding Remarks; References; Chapter 9. Uses of Enucleated Cells; I. Introduction; II. The Technology of Enucleation; III. Uses and Properties of Enucleated Cells; IV. Summary; References; Chapter 10. Enucleation of Somatic Cells with Cytochalasin B; I. Introduction; II. Enucleation of Somatic Cells Attached to the Inner Surface of Ultracentrifuge Nitrocellulose Tubes; III. Enucleation of Somatic Cells on Plastic Coverslips 
505 8 |a IV. Results and DiscussionReferences; Chapter 11. Isolation of Mammalian Heterochromatin and Euchromatin; I. Introduction; II. Isolation of Nuclei; III. Isolation of Heterochromatin and Euchromatin; IV. Conclusion; References; Chapter 12. Measurements of Mammalian Cellular DNA and Its Localization in Chromosomes; I. Introduction; II. Banding Techniques; III. Flow Microfluorometric Analysis; IV. Applications of Methods to Cells in Culture; V. Summary; References; Chapter 13. Large-Scale Isolation of Nuclear Membranes from Bovine Liver; I. Introduction 
520 |a METHODS IN CELL BIOLOGY,VOLUME 8. 
546 |a English. 
650 0 |a Cytology  |x Technique. 
650 0 |a Biology  |x Technique. 
650 2 |a Cytological Techniques  |0 (DNLM)D003584 
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650 7 |a Biologia celular.  |2 larpcal 
700 1 |a Prescott, David M.  |q (David Martin),  |d 1948- 
776 0 8 |i Print version:  |t Methods in cell biology.  |d New York : Academic Press, 1974  |w (DLC) 74641206 
830 0 |a Methods in cell biology ;  |v v. 8. 
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