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Methods in cell biology. Volume 40, A practical guide to the study of calcium in living cells /
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in cell biology ;
v. 40. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Methods in Cell Biology: A Practical Guide to the Study of Calcium in Living Cells; Copyright Page; Contents; Contributors; Preface; Part I: Ca2+ Buffers and [Ca2+]i Perturbation Techniques; Chapter 1. A Practical Guide to the Preparation of Ca2+ Buffers; I. Introduction; II. Which Ca2+ Buffer Should You Use?; III. Basic Mathematical Relationships; IV. Temperature, Ionic Strength, and pH Corrections; V. Ca2+ Measurement and Calibration Solutions; VI. Preparing the Buffer Solution; VII. MaxChelator (MAXC v6.50): An Easy-to-Use Computer Program for Ca2+ Buffer Preparation
- V. Potential Applications of Intracellular Ca2+ Pump Inhibitors in Calcium Cell BiologyReferences; Part II: Microelectrode Techniques for Measuring [Ca2+]i and Ca2+ Fluxes; Chapter 4. How to Make and to Use Calcium-Specific Mini- and Microelectrodes; I. Introduction; II. General Considerations; III. Minielectrodes; IV. Microelectrodes; V. Troubleshooting; References; Chapter 5. The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux; I. Introduction; II. Principles of Vibrating Electrodes; III. Construction of Ion-Selective Probes
- IV. Calibration and SensitivityV. Data Acquisition; VI. Data Analysis and Examples; References; Chapter 6. Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels; I. Introduction; II. Pipettes and Solutions; III. Patch Configurations; IV. Reconstitution of Purified Channel Proteins; V. Expression Cloning of Channel Subunits; VI. Pancreatic � Cell: A Model System for Analysis of Ca2+ Signaling; References; Part III: Fluorescence Techniques for Imaging [Ca2+]i; Chapter 7. Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators; I. Introduction
- II. Fluorescent Ca2+ IndicatorsIII. Loading Indicators into Cells; IV. Manipulation of [Ca2+]; V. Conversion of Indicator Fluorescence Signal into Values of [Ca2+]i; VI. Concluding Remarks; References; Chapter 8. Rapid Simultaneous Estimation of Intracellular Calcium and pH; I. Introduction; II. Simultaneous lntracellular Calcium and pH Measurements; III. Correction Procedures and Calculations; IV. Choosing Calcium and pH Indicator Dyes; V. Possible (but untried) Dye Combinations; VI. Equipment; VII. Results; References