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Methods in cell physiology. Volume 5 /
METHODS IN CELL BIOLOGY,VOLUME 5.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1972.
|
| Colección: | Methods in cell physiology ;
v. 5. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Contents; List of Contributors; Preface; Contents of Previous Volumes; Chapter 1. Procedures for Mammalian Chromosome Preparations; I. Introduction; II. Animal Materials; III. Tissue and Cell Cultures; IV. Cytological Preparations; Chapter 2. Cloning of Mammalian Cells; I. Introduction; II. Theoretical Considerations; III. Procedures for Establishing Clonal Populations; Appendix A. Preparation of Medium F12M; Appendix B. Preparation of Enzyme Solutionss; Appendix C. Cleaning of Glassware; Appendix D. Incubation; References
- Chapter 3. Cell Fusion and Its Application to Studies on the Regulation of the Cell CycleI. Introduction; II. Types of Cell Fusion; III. Viruses and Cell Fusion; IV. Methods of Cell Fusion; V. Preparation of Some Specialized Cells for Fusion; VI. Methods for the Selection and Isolation of Hybrids; VII. Preparation of Fused Cells for Microscopic Examination; VIII. Synchronization of HeLa Cells for Fusion; IX. Application of Cell Fusion to the Study of Events Related to Cell Cycle; X. Conclusions; References; Chapter 4. Marsupial Cells in Vivo and in Vitro; I. Introduction
- II. Karyotypes of MarsupialsIII. The Marsupial Cell Cycle; IV. Tissue Culture of Marsupial Cells; V. Chromosome Synthetic Activities and Cytogenetics of Karyotypically Simple Cells; VI. Concluding Remarks; References; Chapter 5. Nuclear Envelope Isolation; I. Introduction; II. Isolation of the Nuclear Envelope from Individual Cells; III. Isolation of the Nuclear Envelope by Cell Nuclei Fractionation; IV. Isolation of the Nuclear Envelope and Nuclear Membranes by Disintegration of Nuclei and Gradient Centrifugation; V. Isolation of the Nuclear Envelope by Salt Extraction at High Ionic Strength
- VI. Partial Isolation of the Nuclear Envelope by Citric Acid or DetergentsVII. Some Properties of Isolated Nuclear Membranes; VIII. Conclusion; References; Addendum; Chapter 6. Macro- and Micro-Oxygen Electrode Techniques for Cell Measurement; I. Introduction; II. Types of Electrode Systems; III. Evaluation and Calibration; IV. Calculation of Oxygen Content; V. Measurements of Cells and Cell Fractions; VI. Microelectrodes; References; Chapter 7. Methods with Tetrahymena; I. Introduction; II. Media; III. Handling the Cells; IV. Effects of Environmental Factors; V. Synchronization
- VI . Isolation of OrganellesVII. Inhibitors; VIII. Radiochemical Procedures; IX. The Cell Cycle; X. Special Techniques; References; Chapter 8. Comparison of a New Method with Usual Methods for Preparing Monolayers in Electron Microscopy Autoradiography; I. Introduction; II. Methods; III. Results and Discussion; References; Chapter 9. Continuous Automatic Cultivation of Homocontinuous and Synchronized Microalgae; I. Introduction; II. Characterization of Different Types of Mass Cultures; III. Organisms and Growth Conditions; IV. Photoelectrically Controlled Automatic Dilution