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Methods in cell biology. Volume 39, Motility assays for motor proteins /
MOTILITY ASSAYS FOR MOTOR PROTEINS.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1993.
|
| Colección: | Methods in cell biology ;
v. 39. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Motility Assays for Motor Proteins; Copyright Page; Contents; Contributors; Preface; Chapter 1. In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction Using Purified Proteins; I. Introduction; II. Preparation of in Vitro Motility Assay Components; III. In Vitro Assay for Myosin Velocity; IV. In Vitro Assay for Myosin Force in the Motility Assay; V. Components of the Optical Trap System; VI. Future Directions; References; Chapter 2. Myosin-Specific Adaptations of the Motility Assay; I. Introduction; II. Description of Equipment
- III. Basic Assay ProcedureIV. Adaptations of the Assay; V. Quantitation of Rate of Actin Filament Sliding; References; Chapter 3. Motility of Myosin I on Planar Lipid Surfaces; I. Introduction; II. Methods; III. Evaluation of the Method; References; Chapter 4. Microtubule and Axoneme Gliding Assays for Force Production by Microtubule Motor Proteins; I. Introduction; II. Microtubule Gliding Assay; III. Polarity of Force Production; References; Chapter 5. Analyzing Microtubule Motors in Real Time; I. Introduction; II. Configuration of the Computer-Video Microscope Setup
- III. The Microtubule Motility AssayIV. Overview of Typical Results Obtained with the Real-Time Motility Assay; V. Conclusions; Addendum; References; Chapter 6. Assays of Axonemal Dynein-Driven Motility; I. Introduction; II. Dark-Field Video Microscopy; III. Procedures and Analysis of ATP-Induced Microtubule Sliding in Flagellar Axonemes; IV. The Second Assay: Dynein-Driven Microtubule Translocation Using Purified Proteins; V. Summary: Future; References; Chapter 7. Preparation of Marked Microtubules for the Assay of the Polarity of Microtubule-Based Motors by Fluorescence Microscopy
- I. IntroductionII. Preparation of Labeled Microtubules; III. Reagents; IV. Preparation of the Bright Microtubule Seeds; V. Preparation of the Polarity-Marked Microtubules; VI. Visualization of Marked Microtubules in Motor Polarity Assays; References; Chapter 8. Expression of Microtubule Motor Proteins in Bacteria for Characterization in in Vitro Motility Assays; I. Introduction; II. Construction of Plasmids for Expression in Bacteria; III. Vectors Used for Expression of Motor Proteins; IV. Expression of Proteins in Escherichia coli
- V. Partial Purification of Proteins from Bacteria for in Vitro Motility AssaysVI. Brief Description of Motility Assays for Testing the Functional Properties of the Expressed Proteins; VII. Microtubule Binding Assays; VIII. Measurement of ATPase Activity as a Determinant of Motor Protein Function; IX. Problems Encountered with Prokaryotic Expression Systems and Some Solutions; References; Chapter 9. Tracking Nanometer Movements of Single Motor Molecules; I. Introduction; II. Movements by Single Motor Molecules; III. Microtubule Movements on Motor-Coated Glass; IV. Conclusion; References