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Methods in cell biology. Volume 39, Motility assays for motor proteins /
MOTILITY ASSAYS FOR MOTOR PROTEINS.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1993.
|
| Colección: | Methods in cell biology ;
v. 39. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Methods in cell biology. |n Volume 39, |p Motility assays for motor proteins / |c edited by Jonathan M. Scholey. |
| 246 | 3 | 0 | |a Motility assays for motor proteins |
| 260 | |a New York : |b Academic Press, |c 1993. | ||
| 300 | |a 1 online resource (xv, 304 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology ; |v v. 39 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Motility Assays for Motor Proteins; Copyright Page; Contents; Contributors; Preface; Chapter 1. In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction Using Purified Proteins; I. Introduction; II. Preparation of in Vitro Motility Assay Components; III. In Vitro Assay for Myosin Velocity; IV. In Vitro Assay for Myosin Force in the Motility Assay; V. Components of the Optical Trap System; VI. Future Directions; References; Chapter 2. Myosin-Specific Adaptations of the Motility Assay; I. Introduction; II. Description of Equipment | |
| 505 | 8 | |a III. Basic Assay ProcedureIV. Adaptations of the Assay; V. Quantitation of Rate of Actin Filament Sliding; References; Chapter 3. Motility of Myosin I on Planar Lipid Surfaces; I. Introduction; II. Methods; III. Evaluation of the Method; References; Chapter 4. Microtubule and Axoneme Gliding Assays for Force Production by Microtubule Motor Proteins; I. Introduction; II. Microtubule Gliding Assay; III. Polarity of Force Production; References; Chapter 5. Analyzing Microtubule Motors in Real Time; I. Introduction; II. Configuration of the Computer-Video Microscope Setup | |
| 505 | 8 | |a III. The Microtubule Motility AssayIV. Overview of Typical Results Obtained with the Real-Time Motility Assay; V. Conclusions; Addendum; References; Chapter 6. Assays of Axonemal Dynein-Driven Motility; I. Introduction; II. Dark-Field Video Microscopy; III. Procedures and Analysis of ATP-Induced Microtubule Sliding in Flagellar Axonemes; IV. The Second Assay: Dynein-Driven Microtubule Translocation Using Purified Proteins; V. Summary: Future; References; Chapter 7. Preparation of Marked Microtubules for the Assay of the Polarity of Microtubule-Based Motors by Fluorescence Microscopy | |
| 505 | 8 | |a I. IntroductionII. Preparation of Labeled Microtubules; III. Reagents; IV. Preparation of the Bright Microtubule Seeds; V. Preparation of the Polarity-Marked Microtubules; VI. Visualization of Marked Microtubules in Motor Polarity Assays; References; Chapter 8. Expression of Microtubule Motor Proteins in Bacteria for Characterization in in Vitro Motility Assays; I. Introduction; II. Construction of Plasmids for Expression in Bacteria; III. Vectors Used for Expression of Motor Proteins; IV. Expression of Proteins in Escherichia coli | |
| 505 | 8 | |a V. Partial Purification of Proteins from Bacteria for in Vitro Motility AssaysVI. Brief Description of Motility Assays for Testing the Functional Properties of the Expressed Proteins; VII. Microtubule Binding Assays; VIII. Measurement of ATPase Activity as a Determinant of Motor Protein Function; IX. Problems Encountered with Prokaryotic Expression Systems and Some Solutions; References; Chapter 9. Tracking Nanometer Movements of Single Motor Molecules; I. Introduction; II. Movements by Single Motor Molecules; III. Microtubule Movements on Motor-Coated Glass; IV. Conclusion; References | |
| 520 | |a MOTILITY ASSAYS FOR MOTOR PROTEINS. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Cells |x Motility. | |
| 650 | 0 | |a Cytoskeletal proteins. | |
| 650 | 6 | |a Cellules |x Motilit�e. |0 (CaQQLa)201-0039282 | |
| 650 | 6 | |a Prot�eines du cytosquelette. |0 (CaQQLa)201-0043893 | |
| 650 | 7 | |a Cells |x Motility |2 fast |0 (OCoLC)fst00850268 | |
| 650 | 7 | |a Cytoskeletal proteins |2 fast |0 (OCoLC)fst00886324 | |
| 700 | 1 | |a Scholey, Jonathan M. | |
| 776 | 0 | 8 | |i Print version: |t Methods in cell biology. Volume 39, Motility assays for motor proteins. |d New York : Academic Press, 1993 |w (DLC) 74641206 |
| 830 | 0 | |a Methods in cell biology ; |v v. 39. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125641395 |z Texto completo |