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Methods in cell biology. Volume 44, Drosophila melanogaster: practical uses in cell and molecular biology /
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
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| Colección: | Methods in cell biology ;
v. 44. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Drosophila melanogaster: Practical Uses in Cell and Molecular Biology; Copyright Page; Contents; Contributors; Preface: Why the Fly?; Part I: Basic Genetic Methodologies; Chapter 1. Sources of Information about the Fly: Where to Look It Up; I. Introduction; II. Technical References; III. Intellectual References; IV. Electronic References; V. Final Remarks; References; Chapter 2. Care and Feeding of Drosophila melanogaster; I. Introduction; II. Tools of the Trade; III. Room and Board; IV. Culturing Drosophila; V. Reading the Runes-Nomenclature; VI. Resources; VII. Summary
- I. IntroductionII. The Drosophila Colony; III. Media Recipes; IV. Materials Used; References; Chapter 6. Isolation and Organ Culture of Imaginal Tissues; I. Perspectives and Applications; II. Mass Isolation of Imaginal Discs; III. In Vitro Culture of Imaginal Discs; IV. Analysis of Biosynthetic Activity; V. Isolation of Other Larval Organs; VI. Summary and Prospects; References; Chapter 7. Mass Isolation of Fly Tissues; I. Introduction; II. Method for Rearing Flies on Liquid Medium; III. Methods for Isolating Specific Cells; IV. Isolation of Specific Stages of Oogenesis
- V. Concluding ObservationsVI. Solutions; References; Chapter 8. Preparation and Analysis of Pure Cell Populations from Drosophila; I. Introduction; II. Purifying Embryonic Cells by Fluorescence-Activated Cell Sorting; III. Culturing and Analysis of Purified Cells; IV. Conclusions; References; Chapter 9. Transformation Techniques for Drosophila Cell Lines; I. Introduction; II. Maintaining and Cloning Cells; III. Transforming Cells; References; Part III: Biochemical Preparation Methods; Chapter 10. Preparation of Drosophila Nuclei; I. Introduction; II. Dechorionation of Embryos
- III. Disruption of EmbryosIV. Isolation of Nuclei; V. Nuclei from Other Developmental Stages; References; Chapter 11. Isolation and Characterization of RNA-Binding Proteins from Drosophila melanogaster; I. Introduction; II. Isolation of RNA-Binding Proteins by Affinity Chromatography; III. Characterization of Putative RNA-Binding Proteins; IV. Discussion; References; Chapter 12. Chromatin Assembly Extracts from Drosophila Embryos; I. General Introduction; II. Extract Preparation; III. Chromatin Assembly Reaction; IV. Analysis of Reconstituted Chromatin