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Methods in cell biology. Volume 44, Drosophila melanogaster: practical uses in cell and molecular biology /
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in cell biology ;
v. 44. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 050 | 4 | |a QL537.D76 |b M4844 1994eb | |
| 082 | 0 | 4 | |a 595.77/4 |2 23 |
| 245 | 0 | 0 | |a Methods in cell biology. |n Volume 44, |p Drosophila melanogaster: practical uses in cell and molecular biology / |c edited by Lawrence S.B. Goldstein, Eric A. Fyrberg. |
| 246 | 3 | 0 | |a Drosophila melanogaster: practical uses in cell and molecular biology |
| 260 | |a San Diego : |b Academic Press, |c �1994. | ||
| 300 | |a 1 online resource (xx, 755 pages) : |b illustrations (some color). | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology ; |v v. 44 | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future. Key Features * Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster * Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Drosophila melanogaster: Practical Uses in Cell and Molecular Biology; Copyright Page; Contents; Contributors; Preface: Why the Fly?; Part I: Basic Genetic Methodologies; Chapter 1. Sources of Information about the Fly: Where to Look It Up; I. Introduction; II. Technical References; III. Intellectual References; IV. Electronic References; V. Final Remarks; References; Chapter 2. Care and Feeding of Drosophila melanogaster; I. Introduction; II. Tools of the Trade; III. Room and Board; IV. Culturing Drosophila; V. Reading the Runes-Nomenclature; VI. Resources; VII. Summary | |
| 505 | 8 | |a I. IntroductionII. The Drosophila Colony; III. Media Recipes; IV. Materials Used; References; Chapter 6. Isolation and Organ Culture of Imaginal Tissues; I. Perspectives and Applications; II. Mass Isolation of Imaginal Discs; III. In Vitro Culture of Imaginal Discs; IV. Analysis of Biosynthetic Activity; V. Isolation of Other Larval Organs; VI. Summary and Prospects; References; Chapter 7. Mass Isolation of Fly Tissues; I. Introduction; II. Method for Rearing Flies on Liquid Medium; III. Methods for Isolating Specific Cells; IV. Isolation of Specific Stages of Oogenesis | |
| 505 | 8 | |a V. Concluding ObservationsVI. Solutions; References; Chapter 8. Preparation and Analysis of Pure Cell Populations from Drosophila; I. Introduction; II. Purifying Embryonic Cells by Fluorescence-Activated Cell Sorting; III. Culturing and Analysis of Purified Cells; IV. Conclusions; References; Chapter 9. Transformation Techniques for Drosophila Cell Lines; I. Introduction; II. Maintaining and Cloning Cells; III. Transforming Cells; References; Part III: Biochemical Preparation Methods; Chapter 10. Preparation of Drosophila Nuclei; I. Introduction; II. Dechorionation of Embryos | |
| 505 | 8 | |a III. Disruption of EmbryosIV. Isolation of Nuclei; V. Nuclei from Other Developmental Stages; References; Chapter 11. Isolation and Characterization of RNA-Binding Proteins from Drosophila melanogaster; I. Introduction; II. Isolation of RNA-Binding Proteins by Affinity Chromatography; III. Characterization of Putative RNA-Binding Proteins; IV. Discussion; References; Chapter 12. Chromatin Assembly Extracts from Drosophila Embryos; I. General Introduction; II. Extract Preparation; III. Chromatin Assembly Reaction; IV. Analysis of Reconstituted Chromatin | |
| 546 | |a English. | ||
| 650 | 0 | |a Drosophila melanogaster. | |
| 650 | 0 | |a Drosophila. | |
| 650 | 6 | |a Drosophila melanogaster. |0 (CaQQLa)201-0047979 | |
| 650 | 6 | |a Drosophiles. |0 (CaQQLa)201-0281173 | |
| 650 | 7 | |a Drosophila |2 fast |0 (OCoLC)fst00898388 | |
| 650 | 7 | |a Drosophila melanogaster |2 fast |0 (OCoLC)fst00898408 | |
| 700 | 1 | |a Goldstein, Lawrence S. B., |d 1956- | |
| 700 | 1 | |a Fyrberg, Eric A. | |
| 776 | 0 | 8 | |i Print version: |t Methods in cell biology. |d San Diego : Academic Press, �1994 |w (DLC) 95111772 |
| 830 | 0 | |a Methods in cell biology ; |v v. 44. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125641456 |z Texto completo |