Methods in cell biology. Volume 18, Chromatin and chromosomal protein research. III /

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METHODS IN CELL BIOLOGY,VOLUME 18: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH III.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Stein, Gary, Stein, Janet, Kleinsmith, Lewis J.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1978.
Colección:Methods in cell biology ; v. 18.
Temas:
Cytology > Technique.
Biology > Technique.
Cytologie > Technique.
Biologie > Technique.
Biology > Technique
Cytology > Technique
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Chromatin and Chromosomal Protein Research III; Copyright Page; Contents; List of Contributors; Preface; Part A: Chromatin Fractionation. II; Chapter 1. Fractionation of Chromatin into Template-Active and Template-lnactive Portions; I. Introduction; II. Criteria for Fractionation; III. Methods for Fractionation; IV. Structure of Template-Active Chromatin; References; Chapter 2. Fractionation of Chromatin by Buoyant Density-Gradient Sedimentation in Metrizamide; I. Advantages of a Nonionic Buoyant-Density Solute; II. Properties of Metrizamide; III. Fractionation of Chromatin
  • IV. ConclusionsReferences; Chapter 3. Dissection of the Eukaryotic Chromosome with Deoxyribonucleases; I. Introduction; II. General Materials and Methods; III. Staphylococcal Nuclease Digestion of Nuclei; IV. Isolation of the Monomeric Nucleosomal Subunit; V. Digestion with Pancreatic DNase I; VI. Digestion of Nuclei with DNase I I; VII. Cleavage of Nuclei and Chromatin with Restriction Endonucleases; References; Chapter 4. Isolation of Amplified Nucleoli from Xenopus Oocytes; I. Introduction; II. Materials; III. Isolation Procedure; IV. Comments; References
  • Chapter 5. Visualization of Chromatin v-BodiesI. Introduction; II. Methods; III. Demonstration; References; Chapter 6. Isolation and Characterization of Chromatin Subunits; I. Introduction; II. Nuclease Digestion of Nuclei or Chromatin; III. Isolation of Core Particle Subunits and Other Nucleoprotein Fragments; IV. Characterization of Core Particle Subunits; Appendix; References; Part B: Immunochemical Analysis of Chromosomal Properties; Chapter 7. Serological Analyses of Histones; I. Introduction; II. Sources of Sera and Procedures for Immunization; III. Serological Assays
  • IV. Applications of Complement Fixation Assays with Antihistone AntiseraV. Conclusions; References; Chapter 8. Immunochemical Analysis of Nonhistone Proteins; I. Introduction; II. Irnmunogenicity of Nonhistone Proteins; III. Choice of Animal for Production of Antihistone Antibody; IV. Immunization Procedure; V. Testing Antigenicity; VI. Immunohistochemical Localization of Nonhistone Proteins; References; Chapter 9. Radioimmunoassay of Nonhistone Proteins; I. Introduction; II. Methods; III. Typical Results; References
  • Chapter 10. Immunofluorescent Techniques in the Analysis of Chromosomal ProteinsI. Introduction; II. Preparation of Antigens and Antisera; III. Characterization of Antiserum by Indirect Imrnunofluorescent Staining of SDS- Polyacrylamide Gels; IV. Chromosome Fixation and Squashing; V. Indirect Immunofluorescent Staining of Polytene Chromosomes; References; Part C: Sequencing of Histones; Chapter 11. Peptide Mapping and Amino Acid Sequencing of Histones; I. Introduction; II. Preparation and Characterization of Histones for Sequence Work; III. Cleavage of Histones into Peptides

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