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Methods in cell biology. Volume 18, Chromatin and chromosomal protein research. III /
METHODS IN CELL BIOLOGY,VOLUME 18: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH III.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1978.
|
| Colección: | Methods in cell biology ;
v. 18. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 082 | 0 | 4 | |a 571.6 |2 23 |
| 245 | 0 | 0 | |a Methods in cell biology. |n Volume 18, |p Chromatin and chromosomal protein research. |n III / |c edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith. |
| 246 | 3 | 0 | |a Chromatin and chromosomal protein research |
| 260 | |a New York : |b Academic Press, |c 1978. | ||
| 300 | |a 1 online resource (xiv, 462 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology ; |v v. 18 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Chromatin and Chromosomal Protein Research III; Copyright Page; Contents; List of Contributors; Preface; Part A: Chromatin Fractionation. II; Chapter 1. Fractionation of Chromatin into Template-Active and Template-lnactive Portions; I. Introduction; II. Criteria for Fractionation; III. Methods for Fractionation; IV. Structure of Template-Active Chromatin; References; Chapter 2. Fractionation of Chromatin by Buoyant Density-Gradient Sedimentation in Metrizamide; I. Advantages of a Nonionic Buoyant-Density Solute; II. Properties of Metrizamide; III. Fractionation of Chromatin | |
| 505 | 8 | |a IV. ConclusionsReferences; Chapter 3. Dissection of the Eukaryotic Chromosome with Deoxyribonucleases; I. Introduction; II. General Materials and Methods; III. Staphylococcal Nuclease Digestion of Nuclei; IV. Isolation of the Monomeric Nucleosomal Subunit; V. Digestion with Pancreatic DNase I; VI. Digestion of Nuclei with DNase I I; VII. Cleavage of Nuclei and Chromatin with Restriction Endonucleases; References; Chapter 4. Isolation of Amplified Nucleoli from Xenopus Oocytes; I. Introduction; II. Materials; III. Isolation Procedure; IV. Comments; References | |
| 505 | 8 | |a Chapter 5. Visualization of Chromatin v-BodiesI. Introduction; II. Methods; III. Demonstration; References; Chapter 6. Isolation and Characterization of Chromatin Subunits; I. Introduction; II. Nuclease Digestion of Nuclei or Chromatin; III. Isolation of Core Particle Subunits and Other Nucleoprotein Fragments; IV. Characterization of Core Particle Subunits; Appendix; References; Part B: Immunochemical Analysis of Chromosomal Properties; Chapter 7. Serological Analyses of Histones; I. Introduction; II. Sources of Sera and Procedures for Immunization; III. Serological Assays | |
| 505 | 8 | |a IV. Applications of Complement Fixation Assays with Antihistone AntiseraV. Conclusions; References; Chapter 8. Immunochemical Analysis of Nonhistone Proteins; I. Introduction; II. Irnmunogenicity of Nonhistone Proteins; III. Choice of Animal for Production of Antihistone Antibody; IV. Immunization Procedure; V. Testing Antigenicity; VI. Immunohistochemical Localization of Nonhistone Proteins; References; Chapter 9. Radioimmunoassay of Nonhistone Proteins; I. Introduction; II. Methods; III. Typical Results; References | |
| 505 | 8 | |a Chapter 10. Immunofluorescent Techniques in the Analysis of Chromosomal ProteinsI. Introduction; II. Preparation of Antigens and Antisera; III. Characterization of Antiserum by Indirect Imrnunofluorescent Staining of SDS- Polyacrylamide Gels; IV. Chromosome Fixation and Squashing; V. Indirect Immunofluorescent Staining of Polytene Chromosomes; References; Part C: Sequencing of Histones; Chapter 11. Peptide Mapping and Amino Acid Sequencing of Histones; I. Introduction; II. Preparation and Characterization of Histones for Sequence Work; III. Cleavage of Histones into Peptides | |
| 520 | |a METHODS IN CELL BIOLOGY,VOLUME 18: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH III. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Cytology |x Technique. | |
| 650 | 0 | |a Biology |x Technique. | |
| 650 | 6 | |a Cytologie |x Technique. |0 (CaQQLa)201-0005040 | |
| 650 | 6 | |a Biologie |x Technique. |0 (CaQQLa)201-0020329 | |
| 650 | 7 | |a Biology |x Technique |2 fast |0 (OCoLC)fst00832494 | |
| 650 | 7 | |a Cytology |x Technique |2 fast |0 (OCoLC)fst00886306 | |
| 700 | 1 | |a Stein, Gary. | |
| 700 | 1 | |a Stein, Janet. | |
| 700 | 1 | |a Kleinsmith, Lewis J. | |
| 776 | 0 | 8 | |i Print version: |t Methods in cell biology. |d New York : Academic Press, 1978 |w (DLC) 74641206 |
| 830 | 0 | |a Methods in cell biology ; |v v. 18. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125641180 |z Texto completo |