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Methods in cell biology. Volume 16, Chromatin and chromosomal protein research. I /
METHODS IN CELL BIOLOGY,VOLUME 16: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH I.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1977.
|
| Colección: | Methods in cell biology ;
16. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 082 | 0 | 4 | |a 571.6 |2 23 |
| 245 | 0 | 0 | |a Methods in cell biology. |n Volume 16, |p Chromatin and chromosomal protein research. |n I / |c edited by Gary Stein and Janet Stein, Lewis J. Kleinsmith. |
| 246 | 3 | 0 | |a Chromatin and chromosomal protein research |
| 260 | |a New York : |b Academic Press, |c 1977. | ||
| 300 | |a 1 online resource (xiii, 494 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology ; |v 16 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Cover; Contents; List of Contributors; Preface; Part A: Isolation of Nuclei and Preparation of Chromatin. I; Chapter 1. Methods for Isolation of Nuclei and Nucleoli; I. Isolation of Nuclei; II. The Sucrose-Calcium Procedure for Isolation of Nuclei; Ill. The Citric Acid Procedure for Isolation of Nuclei; IV. The Use of Detergents for Nuclear Isolation; V. Isolation and Purification of Nucleoli; VI. The Sonication Procedure for Isolation of Nucleoli; VII. Compression-Decompression Procedure for Isolation of Nucleoli; VIII. ""Nucleolar"" Preparations Obtained by Chemical Extractions; References | |
| 505 | 8 | |a Chapter 2. Nonaqueous Isolation of Nuclei from Cultured CellsI. Introduction; II. Lyophilization Apparatus; III. Cell Culture; IV. Preparation of Cells for Freezing; V. Freezing Cells; VI . Drying Cells; VII. Homogenization; VIII. Separation of Cell Fractions; IX. Methods of Handling Glycerol Suspensions; X. Appearance of Purified Nuclei; XI. Recovery of Nuclear DNA; XII. Distributions of RNA Species; XIII. Electrophoresis of Nuclear and Cytoplasmic Proteins; XIV. ATP Content of Whole Cells and of Isolated Nuclei; XV. Conclusions; References | |
| 505 | 8 | |a Chapter 3. Isolation of Nuclei Using Hexylene GlycolI. Introduction; II. General Methods; III. Specific Nuclear Isolation Methods; IV. Nuclear Characterization; V. Discussion; References; Chapter 4. Isolation of Nuclei and Preparation of Chromatin from Plant Tissues; I. Introduction; II. Isolation of Nuclei and Chromatin from Maize Tissues; III. Concluding Remarks; IV. List of Buffers; Note Added in Proof; References; Chapter 5. Methods for the Isolation of Nuclei from Ciliated Protozoans; I. Introduction; II. Isolation Procedures; III. Other Criteria for Selection of Method | |
| 505 | 8 | |a IV. Conclusions and PerspectivesReferences; Chapter 6 . The Isolation of Nuclei from Fungi; I. Introduction; II. Isolation of Nuclei from Physarum polycephalum; III. Isolation of Nuclei from Neurospora crussu; IV. Conclusion; References; Part B: Nuclear-Cytoplasmic Exchange; Chapter 7. Methods for Nuclear Transplantation in Amphibia; I. Introduction; II. The Preparation of Nuclei for Injection; III. Enucleation; IV. The Transplantation Procedure; V. Culture; VI. Labeling; VII. Applications of Nuclear Transplantation Techniques in Amphibia to the Study of Chromosomal Proteins | |
| 505 | 8 | |a Note Added in ProofReferences; Chapter 8. Methods for Studying Nucleocytoplasmic Exchange of Nonhistone Proteins in Embryos; I. Introduction; II. Materials; III. Procedures for Obtaining Embryos and Oocytes; IV. Radioactive Labeling of Embryos and Oocytes; V. Nucleocytoplasmic Exchange of Nonhistones; VI. Cytological Procedures; VII. Concluding Remarks; References; Chapter 9. Methods for Studying Nucleocytoplasmic Exchange of Macromolecules; I. Introduction; II. Methods; III. Results; IV. Conclusions and Applications; References; Part C: Fractionation and Characterization of Histones. I | |
| 520 | |a METHODS IN CELL BIOLOGY,VOLUME 16: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH I. | ||
| 546 | |a English. | ||
| 650 | 0 | |a Cytology |x Technique. | |
| 650 | 0 | |a Biology |x Technique. | |
| 650 | 6 | |a Cytologie |x Technique. |0 (CaQQLa)201-0005040 | |
| 650 | 6 | |a Biologie |x Technique. |0 (CaQQLa)201-0020329 | |
| 650 | 7 | |a Biology |x Technique |2 fast |0 (OCoLC)fst00832494 | |
| 650 | 7 | |a Cytology |x Technique |2 fast |0 (OCoLC)fst00886306 | |
| 700 | 1 | |a Stein, Gary. | |
| 700 | 1 | |a Stein, Janet. | |
| 700 | 1 | |a Kleinsmith, Lewis J. | |
| 776 | 0 | 8 | |i Print version: |t Methods in cell biology. Volume XVI, Chromatin and chromosomal protein research. I. |d New York : Academic Press, 1977 |w (DLC) 74641206 |
| 830 | 0 | |a Methods in cell biology ; |v 16. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125641166 |z Texto completo |