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Stem cell culture /
Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this b...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
2008.
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| Edición: | 1st ed. |
| Colección: | Methods in cell biology ;
v. 86. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; Stem Cell Culture; Copyright Page; Contents; Contributors; Preface; Biography; Chapter 1: Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions; I. Introduction; II. Methods; III. Discussion; IV. Materials; References; Chapter 2: Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions; I. Introduction; II. Methods and Materials; III. Results; IV. Discussion and Summary; References; Chapter 3: Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation
- I. IntroductionII. Rationale; III. Methods; IV. Summary; References; Chapter 4: Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Discussion; References; Chapter 5: Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability; I. Introduction; II. Amniotic Fluid Cell Composition and Stem Cells; III. In Vitro Potential of Amniotic Fluid Stem Cells; IV. Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells; V. Discussion; References
- Chapter 6: Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical CordI. Introduction; II. Rationale; III. Methods; IV. Materials; V. UCMSCs and Culture Characteristics; VI. Discussion; References; Chapter 7: Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs); I. Introduction; II. Methods; III. Concluding Remarks; References; Chapter 8: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance; I. Introduction; II. Epithelial-Mesenchymal Relationship; III. Stem Cells and Lineage Biology
- IV. Liver ProcessingV. Fractionation of Liver Cell Subpopulations; VI. Feeder Cells; VII. Extracellular Matrix; VIII. Culture Media; IX. Monolayer Cultures of Cells; X. Three-Dimensional Systems; Appendix 1: Nomenclature, Glossary, and Abbreviations; Appendix 2: Details on the Preparation of Buffers/Reagents; Appendix 3: Commercial Sources of Reagents; Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells; References; Chapter 9: Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo; I. Introduction
- II. RationaleIII. Methods; IV. Materials; V. Discussion; VI. Summary; References; Chapter 10: Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Discussion; VI. Summary; References; Chapter 11: Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue; I. Introduction; II. Isolation of hMSCs from Trabecular Bone; III. Isolation of hMSCs from Adipose Tissue; IV. Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs; V. Surface Marker Characterization of hMSCs