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Chemokines /
The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusin...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California :
Academic Press/Elsevier,
[2009]
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| Edición: | 1st ed. |
| Colección: | Methods in enzymology ;
v. 460. Methods in enzymology ; v. 461. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Part A (v. 460)
- Part B (v. 461).
- Front Cover; Methods In Enzymology: Chemokines, Part A; Copyright Page; Contents; Contributors; Preface; Section 1: Chemokines and Receptors in Disease; Chapter 1: Chapter One Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects ; 1. Introduction; 2. Modifying Chemokine Expression in Breast Tumor Cells; 3. Establishment of Primary Local Breast Tumors and Pulmonary Metastases; Acknowledgments; References; Chapter 2: Chapter 2 CCR5 Pharmacology Methodologies and Associated Applications; 1. Introduction.
- 2. CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Ag3. CCR5-Associated Ligand-Binding Assays; 4. Surrogate In Vitro Antiviral Assays; 5. CCR5 Site-Directed Mutagenesis and Ligand Docking Studies; 6. Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice; References; Chapter 3: Chapter Three CXCR4 and Mobilization of Hematopoietic Precursors; 1. Introduction; 2. HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis; 3. Donor Selection for HSPC Mobilization; 4. Flow Cytometric Enumeration of Mobilized HSPCs.
- 5. Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor6. Flow Cytometric Analysis of CXCR4 Expression on Human CD34+ Subsets; 7. Functional Characterization of Mobilized HSPCs; 8. Concluding Remarks; Acknowledgment; References; Chapter 4: Double-Label Nonradioactive ; 1. Introduction; 2. Basic Protocol for ISH (Using Digoxygenin-Labeled Probe); 3. Comments; References; Chapter 5: Chapter Five Expression of Chemokines and Chemokine Receptors in Human Colon Cancer; 1. Introduction; 2. Materials and Methods; 3. Results; 4. Discussion; Acknowledgments; References.
- Section II: Chemokine Related Proteins from PathogensChapter 6: Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation; 1. Introduction; 2. Cloning of vGPCR; 3. Assaying vGPCR Transforming Activity In Vitro; 4. vGPCR Transforming Activity In Vivo Using Xenograft Systems; 5. In Vivo Targeted Infection Using the TVA-RCAS System; 6. vGPCR-Induced Paracrine Transformation; 7. Characterization of vGPCR-Induced Molecular Signaling; 8. Evaluation of Activation of Second-Messenger-Generating Systems.
- 9. Activation of Signal-Transducing Protein Kinases and Small GTPases10. Akt Kinase Assay; 11. vGPCR Stimulated Activation of Rac1-Pulldown Assays; 12. Western Blotting Using Phospho-Specific Antibodies; 13. Activation of Transcription Factors; 14. Global Changes in Gene Expression: Microarray Analysis; 15. NFkappaB Luciferase Assays; 16. NFkappaB Binding Assays; 17. Nuclear Translocation of NFkappaB; Acknowledgments; References; Chapter 7: Chapter 7 Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Recept; 1. Introduction.