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Single molecule tools. Part A, Fluorescence based approaches /
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme as...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, Calif :
Academic Press/Elsevier,
2010.
|
| Colección: | Methods in enzymology ;
v. 472. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
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| 245 | 0 | 0 | |a Single molecule tools. |n Part A, |p Fluorescence based approaches / |c edited by Nils G. Walter. |
| 260 | |a San Diego, Calif : |b Academic Press/Elsevier, |c 2010. | ||
| 300 | |a 1 online resource (l, 476 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 472 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 505 | 0 | 0 | |t Star polymer surface passivation for single-molecule detection / |r J�urgen Groll and Martin Moeller -- |t Azide-specific labeling of biomolecules by Staudinger-Bertozzi ligation: phosphine derivatives of fluorescent probes suitable for single-molecule fluorescence spectroscopy / |r Anirban Chakraborty [and others] -- |t Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast / |r John Abelson, Haralambos Hadjivassiliou and Christine Guthrie -- |t Nanovesicle trapping for studying weak protein interactions by single-molecule FRET / |r Jaime J. Ben�itez, Aaron M. Keller and Peng Chen -- |t Droplet confinement and fluorescence measurement of single molecules / |r Lori S. Goldner, Ana M. Jofre and Jianyong Tang -- |t Single-molecule fluorescence spectroscopy using phospholipid bilayer nanodiscs / |r Abhinav Nath [and others] -- |t Single-molecule spectroscopy using microfluidic platforms / |r Samuel Kim and Richard N. Zare -- |t Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning / |r Julian Weghuber [and others] -- |t Analysis of complex single-molecule FRET time trajectories / |r Mario Blanco and Nils G. Walter -- |t Single-molecule fluorescence studies of intrinsically disordered proteins / |r Allan Chris M. Ferreon [and others] -- |t Measuring the energetic coupling of tertiary contacts in RNA folding using single molecule fluorescence resonance energy transfer / |r Max Greenfield and Daniel Herschlag -- |t A highly purified, fluorescently labeled in vitro translation system for single-molecule studies of protein synthesis / |r Jingyi Fei [and others] -- |t Watching individual proteins acting on single molecules of DNA / |r Ichiro Amitani [and others] -- |t DNA curtains for high-throughput single-molecule optical imaging / |r Eric C. Greene [and others] -- |t Scanning FCS for the characterization of protein dynamics in live cells / |r Zden�ek Petr�a�sek, Jonas Ries and Petra Schwille -- |t Observing protein interactions and their stoichiometry in living cells by brightness analysis of fluorescence fluctuation experiments / |r Yan Chen [and others] -- |t Detection of individual endogenous RNA transcripts in situ using multiple singly labeled probes / |r Arjin Raj and Sanjay Tyagi -- |t Single mRNA tracking in live cells / |r Hye Yoon Park, Adina R. Buxbaum and Robert H. Singer -- |t Single-molecule sequencing: sequence methods to enable accurate quantitation / |r Christopher Hart [and others] -- |t Real-time DNA sequencing from single polymerase molecules / |r Jonas Korlach [and others]. |
| 520 | |a Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines | ||
| 650 | 0 | |a Fluorescence spectroscopy |v Congresses. | |
| 650 | 0 | |a Fluorescent probes |v Congresses. | |
| 650 | 0 | |a Luminescence spectroscopy |v Congresses. | |
| 650 | 0 | |a Luminescent probes |v Congresses. | |
| 650 | 0 | |a Biosensors |v Congresses. | |
| 650 | 0 | |a Fluorescence spectroscopy. | |
| 650 | 0 | |a Fluorescence microscopy. | |
| 650 | 0 | |a Molecular probes. | |
| 650 | 1 | 2 | |a Spectrometry, Fluorescence |0 (DNLM)D013050 |
| 650 | 2 | 2 | |a Microscopy, Fluorescence |0 (DNLM)D008856 |
| 650 | 2 | 2 | |a Molecular Probes |0 (DNLM)D015335 |
| 650 | 6 | |a Spectroscopie de fluorescence |0 (CaQQLa)201-0066421 |v Congr�es. |0 (CaQQLa)201-0378219 | |
| 650 | 6 | |a Sondes fluorescentes |0 (CaQQLa)201-0250401 |v Congr�es. |0 (CaQQLa)201-0378219 | |
| 650 | 6 | |a Spectroscopie de luminescence |0 (CaQQLa)201-0044100 |v Congr�es. |0 (CaQQLa)201-0378219 | |
| 650 | 6 | |a Sondes luminescentes |0 (CaQQLa)000259995 |v Congr�es. |0 (CaQQLa)201-0378219 | |
| 650 | 6 | |a Biocapteurs |0 (CaQQLa)201-0165323 |v Congr�es. |0 (CaQQLa)201-0378219 | |
| 650 | 6 | |a Spectroscopie de fluorescence. |0 (CaQQLa)201-0066421 | |
| 650 | 6 | |a Microscopie de fluorescence. |0 (CaQQLa)201-0014904 | |
| 650 | 6 | |a Sondes mol�eculaires. |0 (CaQQLa)201-0017000 | |
| 650 | 7 | |a fluorescence microscopy. |2 aat |0 (CStmoGRI)aat300252489 | |
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| 650 | 7 | |a Fluorescence microscopy |2 fast |0 (OCoLC)fst00928103 | |
| 650 | 7 | |a Biosensors |2 fast |0 (OCoLC)fst00832703 | |
| 650 | 7 | |a Fluorescence spectroscopy |2 fast |0 (OCoLC)fst00928106 | |
| 650 | 7 | |a Fluorescent probes |2 fast |0 (OCoLC)fst00928131 | |
| 650 | 7 | |a Luminescence spectroscopy |2 fast |0 (OCoLC)fst01003662 | |
| 650 | 7 | |a Luminescent probes |2 fast |0 (OCoLC)fst01003664 | |
| 653 | 0 | 0 | |a fluorescentie |
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| 653 | 0 | 0 | |a laser fluorescence spectroscopy |
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| 653 | 0 | 0 | |a moleculen |
| 653 | 0 | 0 | |a molecules |
| 653 | 1 | 0 | |a Proteins and Enzymes |
| 653 | 1 | 0 | |a Eiwitten en enzymen |
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| 700 | 1 | |a Walter, Nils G. | |
| 830 | 0 | |a Methods in enzymology ; |v v. 472. | |
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| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879 |z Texto completo |