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Basic techniques for transmission electron microscopy /
Basic Techniques For Transmission Electron Microscopy.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Orlando :
Academic Press,
1986.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Basic Techniques for Transmission Electron Microscopy; Copyright Page; Table of Contents; Preface; Chapter 1. Chemical Fixation; INTRODUCTION; HAZARDS, PRECAUTIONS, AND SAFE HANDLING OF REAGENTS; FIXATIVES; PREPARATION OF BUFFERS; PREPARATION OF TISSUE BLOCKS; FIXATION; MICROWAVE FIXATION; ARTIFACTUAL ELECTRON-DENSE GRANULES OCCURRING DURING DOUBLE FIXATION; Chapter 2. Rinsing, Dehydration, and Embedding; INTRODUCTION; STANDARD PROCEDURE FOR FIXATION, RINSING, DEHYDRATION, AND EMBEDDING; GRADUAL, PROGRESSIVE DEHYDRATION AND EMBEDDING
- PROCEDURE FOR MINIMIZING CHEMICAL HAZARDS DURING SPECIMEN PREPARATIONLOW-TEMPERATURE INFILTRATION AND EMBEDDING; INCOMPLETE DEHYDRATION FOR LIPID PRESERVATION; WATER-IMMISCIBLE EMBEDDING MEDIA; WATER-MISCIBLE EMBEDDING MEDIA; MIXED-RESIN EMBEDDING MEDIA; EMBEDDING; Chapter 3. Sectioning; INTRODUCTION; EXAMINATION OF GLASS KNIVES; DIAMOND KNIVES; GRIDS; SPECIMEN BLOCK TRIMMING; PREPARATION OF TROUGHS; MOUNTING THE SPECIMEN BLOCK AND KNIFE; SECTIONING PROCEDURE; DEFECTS APPEARING DURING SECTIONING; SERIAL SECTIONING; SPECIFIC METHODS; SEMITHIN SECTIONING; CRYOFIXATION AND CRYOULTRAMICROTOMY
- Chapter 4. Positive StainingINTRODUCTION; STAINS; LIPID PRESERVATION AND STAINING; MULTIPLE STAINING; DOUBLE STAINING OF THIN SECTIONS WITH URANYL ACETATE AND LEAD CITRATE; MULTIPLE
- GRID STAINING; REMOVAL OF BOUND OSMIUM FROM THIN SECTIONS; STAINING FOR HIGH-VOLTAGE ELECTRON MICROSCOPY; PRECAUTIONS TO MINIMIZE ARTIFACTUAL STAINING PRECIPITATES; REMOVAL OF ARTIFACTUAL STAINING PRECIPITATES FROM THIN SECTIONS; REMOVAL OF EPOXY RESINS FROM SEMITHIN SECTIONS BEFORE STAINING; STAINING METHODS FOR SEMITHIN SECTIONS; Chapter 5. Negative Staining; INTRODUCTION; NEGATIVE STAINS
- WETTABILITY OF SUPPORT FILMSGENERAL METHODS; VIRUSES; SPECIFIC METHODS; Chapter 6. Support Films; INTRODUCTION; PLASTIC SUPPORT FILMS; CARBON SUPPORT FILMS; SUBSTRATES FOR CARBON EVAPORATION; PERFORATED FILMS; Chapter 7. Specific Preparation Methods; ACTIN FILAMENTS; ALGAE (UNICELLULAR): GENERAL METHOD; ALGAE (GREEN); ALGAE (RED); AMOEBA; ANTHERS; ATTACHMENT OF CELLS TO SUBSTRATUM; BACTERIA; BACTERIAL COLONIES; BACTERIAL MICROCOLONIES GROWN ON SOLID SURFACES; BONE (Undecalcified); BUFFY COAT; CARTILAGE; CELL COLONIES GROWN ON SOFT AGAR; CELLS ATTACHED TO GLASS SURFACES; CELLS IN CULTURE
- CELL LAYERSCELL MONOLAYERS; CELL SUSPENSIONS (Mereau et al., 1982); CEREBROSPINAL FLUID CELLS (Lutke
- Schipholt and Stadhouders, 1981); CHROMOSOMES; CILIATES; CREAM CHEESE; CYSTS; DIATOMS; ECHINODERMS (Dietrich and Fontaine, 1975); EGGS AND ZYGOTES; EFFUSION FLUID; EMBRYOS (e.g., Chick); EPITHELIUM (e.g., Cheek Pouch); ERYTHROCYTES (e.g., Avian); EXAMINATION OF SPECIFIC PARTS OF IDENTIFIED NEURONS (SOMATOSENSORY CORTEX); EXTRACELLULAR PROTOPLASTS IN SUSPENSION; EYES; FETAL TISSUE (e.g., Pig Liver and Tooth Germs); FINE-NEEDLE ASPIRATION BIOPSY; FLAGELLATES; FREE CELLS; FUNGI; HeLa CELLS