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Practical protein crystallography /

Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and c...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: McRee, Duncan Everett, 1957-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1993.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Laboratory techniques
  • Preparing protein samples
  • Protein crystal growth
  • Crystal storage and handling
  • Crystal soaking
  • Anaerobic crystals
  • Data collection techniques
  • Preparing crystals for data collection
  • Optical alignment
  • X-ray sources
  • Preliminary characterization
  • Heavy-atom derivative scanning with film
  • Overall data-collection strategy
  • Overview of older film techniques
  • Four-circle diffractometer data collection
  • Area-detector data collection
  • Image-plate data collection
  • Synchrotron-radiation light sources
  • Data reduction
  • Computational techniques
  • Terminology
  • Basic computer techniques
  • Data reduction and statistical analysis
  • Patterson synthesis
  • Fourier techniques
  • Isomorphous replacement phasing
  • Molecular replacement
  • Non-crystallographic symmetry
  • Density modification
  • Multi-wavelength with anomalous scattering
  • Refinement of coordinates
  • Fitting of maps
  • Analysis of coordinates
  • Protein crystallography cookbook
  • Multiple isomorphous replacement
  • Mutant studies
  • Substrate-analog example.
  • (cont) Molecular replacement
  • Crystallographic equations in computer code
  • XtalView users' guide
  • Reference section
  • XtalView programmers' guide.