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Practical capillary electrophoresis /
High performance capillary electrophoresis (HPCE) is the newest and perhaps most powerful separation technique available today. This single-authored text provides an integrated, comprehensive, and clearly illustrated look at the field. Users of HPCE will gain a basic understanding of principles unde...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Boston :
Academic Press,
�1993.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Introduction
- Electrophoresis
- Microchromatographic separation methods
- Capillary electrophoresis
- Historical perspective
- Instrumentation
- Sources of information on HPCE
- Capillary electrophoresis: a family ot techniques
- Basic concepts
- Electrical conduction in fluid solution
- Language of electrophoresis
- Electroendoosmosis
- Efficiency
- Resolution
- Joule heating
- Optimizing the voltage and temperature
- Capillary diameter and buffer ionic strength
- Optimizing the capillary length
- Buffers
- Capillaries
- Capillary zone electrophoresis
- Mobility
- Buffer additives
- Solute-wall interactions
- Bandbroadening
- Applications
- Methods development
- Capillary isoelectric focusing
- Capillary coatings and electroosmotic flow
- Performing a run
- Focusing and mobilization problems
- Applications
- Size separations in capillary gels and polymer networks
- Separation mechanism
- Materials for CGE
- Applications
- Pulsed-field capillary electrophoresis
- Future of CGE
- Capillary isotachophoresis
- Separation mechanism
- Instrumental aspects
- Buffer selection.
- (cont) Advantages and problems with CITP
- Applications
- Electrokinetic capillary chromatography
- Micelles
- Separation mechanism
- Sources of bandbroadening
- Selecting the electrolyte system
- Elution range of MECC
- Alternative surfactant systems
- Applications and methods development
- Cyclodextrins
- Chiral recognition
- Optimization
- Capillary electrochromatography
- Electroosmotic pump
- Bandbroadening
- Applications
- Electrochromatography in perspective
- Injection
- Volumetric constraints on injection size
- Performing an injection and run
- Injection techniques
- Injection problems and solutions
- Alternative injection schemes
- Stacking
- Other enrichment techniques
- Detection
- On-capillary detection
- Detection problem
- Classes of detectors
- Limits of detection
- Bandbroadening
- Absorption detection
- Fluorescence detection
- Indirect detection
- Mass spectrometry
- Other detection techniques
- Fraction collection
- Putting it all together
- Selecting the mode of HPCE
- Requirements for robust separations.
- (cont) Realistic compromises
- Linear dynamic range
- Sample preparation
- Quantitative analysis
- Correction factors
- Special topics
- Field effect electroendoosmosis
- Ultrafast separations
- HPCE on a chip
- Multiple capillary systems
- Gradient and programmed systems
- Two-dimensional systems
- Immunologic trace enrichment.