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Regulators and effectors of small GTPases : Rho family /

The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission....

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Balch, William Edward, 1949- (Editor ), Der, Channing J. (Editor ), Hall, A. (Alan) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego, California : Elsevier Academic Press, �2006.
Colección:Methods in enzymology ; v. 406.
Temas:
Acceso en línea:Texto completo
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MARC

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035 |a (OCoLC)441804750  |z (OCoLC)63760063  |z (OCoLC)171131353  |z (OCoLC)436734147  |z (OCoLC)648300196  |z (OCoLC)815530246  |z (OCoLC)823109013  |z (OCoLC)823829539  |z (OCoLC)823899243  |z (OCoLC)824090516  |z (OCoLC)824138109  |z (OCoLC)846753053  |z (OCoLC)1125522173 
050 4 |a QP601  |b .C57 v. 406 
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084 |a 35.70  |2 bcl 
245 0 0 |a Regulators and effectors of small GTPases :  |b Rho family /  |c edited by William E. Balch, Channing J. Der, Alan Hall. 
260 |a San Diego, California :  |b Elsevier Academic Press,  |c �2006. 
300 |a 1 online resource (xliii, 798 pages, 24 unnumbered pages of plates) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v v. 406 
504 |a Includes bibliographical references and indexes. 
505 0 |a Purification and Biochemical Properties of Rac1, 2, 3 and the Splice Variant Rac1b -- Biochemical Analyses of the Wrch Atypical Rho Family GTPases -- Purification of P-Rex1 from Neutrophils and Nucleotide Exchange Assay -- In Vitro Guanine Nucleotide Exchange Activity of DHR-2/DOCKER/CZH2 Domains -- Biochemical Characterization of the Cool (Cloned-Out-of-Library)/Pix (Pak-Interactive Exchange Factor) Proteins -- GEF and Glucosylation Assays on Liposome-Bound Rac -- Phosphorylation of RhoGDI by p21-activated kinase 1 -- Purification of ARAP3 and Characterization of GAP Activities -- Regulation of RhoGAP specificity by Phospholipids and Prenylation -- Purification and Activity of the Rho ADP-ribosylating binary C2/C3 Toxin -- Purification of TAT-C3 Exoenzyme -- Imaging and photobleach correction of Mero-CBD, Sensor of Endogenous Cdc42 Activation -- Cdc42 and PI(4,5)P2-induced actin Assembly in Xenopus Egg Extracts -- In vitro reconstitution of Cdc42-mediated actin assembly using purified components -- Biochemical analysis of mammalian formin effects on actin Dynamics -- Formin proteins: purification and measurement of effects on actin assembly -- Purification and kinase assay of PKN -- Purification and enzyme activity of ACK1 -- Direct Activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid Vesicles -- Regulation of PLCbeta isoforms by Rac -- Biochemical properties and Inhibitors of (N- )WASP -- The Use of GFP to localize Rho GTPases in living cells -- Analysis of the spatiotemporal activation of Rho GTPases using Raichu probes -- Measurement of activity of Rho GTPases during mitosis -- Inhibition of Rho GTPases by RNA interference -- RNA interference techniques to study epithelial cell adhesion and polarity -- Nucleofection of Primary Neurons -- Dock180-ELMO cooperation in Rac activation -- Rho GTPase activation by cell-cell adhesion -- Activation of Rap1, Cdc42, and Rac by nectin adhesion system -- Analysis of activated GAPs and GEFs in cell lysates -- Degradation of RhoA by Smurf1 ubiquitin ligase -- Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin -- Regulation of superoxide-producing NADPH oxidases in nonphagocytic cells -- Activation of MEKK1 by Rho GTPases -- Activation of the apoptotic JNK pathway through the Rac1-binding scaffold protein POSH -- Quantification of isozyme-specific activation of phospholipase C-beta2 by Rac GTPases and phospholipase C-epsiv by Rho GTPases in an intact cell assay system -- Activation of Rho and Rac by Wnt/frizzled signaling -- Fluorescent assay of cell-permeable C3 transfease activity -- Use of TIRF microscopy to visualize actin and microtubules in migrating cells -- Inhibition of ROCK by RhoE -- Conditional Regulation of a ROCK-estrogen receptor fusion protein -- Rational design and applications of a Rac GTPase-specific small molecule Inhibitor -- In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization -- Real-time centrosome reorientation during fibroblast migration -- Lentiviral delivery of RNAi in hippocampal neurons -- Methods for studying neutrophil chemotaxis -- Measurement of epidermal growth factor receptor turnover and effects of Cdc42 -- Tumor cell migration in three dimensions -- An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration -- Analysis of a mitotic role of Cdc42 -- Plexin-induced collapse assay in COS cells -- Morphological and biochemical analysis of Rac1 in three-dimensional epithelial cell cultures -- Using three-dimensional acinar structures for molecular and cell biological assays -- TC10 and insulin-stimulated glucose transport -- GTPases and the control of neuronal polarity -- In vitro assembly of filopodia-like bundles. 
520 |a The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission. These GTPases act as molecular switches and their activities are controlled by a large number of regulatory molecules that affect either GTP loading (guanine nucleotide exchange factors or GEFs) or GTP hydrolysis (GTPase activating proteins or GAPs). 
588 0 |a Print version record. 
546 |a English. 
650 0 |a Guanosine triphosphatase. 
650 0 |a Rho GTPases. 
650 1 2 |a rho GTP-Binding Proteins  |x physiology  |0 (DNLM)D020741Q000502 
650 2 |a GTP Phosphohydrolases  |0 (DNLM)D020558 
650 2 |a rho GTP-Binding Proteins  |0 (DNLM)D020741 
650 6 |a GTPases.  |0 (CaQQLa)201-0245840 
650 6 |a GTPases rho.  |0 (CaQQLa)201-0439304 
650 7 |a SCIENCE  |x Life Sciences  |x Molecular Biology.  |2 bisacsh 
650 7 |a Guanosine triphosphatase  |2 fast  |0 (OCoLC)fst00948750 
650 7 |a Rho GTPases  |2 fast  |0 (OCoLC)fst01097143 
650 7 |a Rab-Proteine  |2 gnd  |0 (DE-588)4508024-0 
650 7 |a Rho-Proteine  |2 gnd  |0 (DE-588)4681926-5 
650 1 7 |a GTPasen.  |2 gtt 
650 1 7 |a Regelmechanismen.  |2 gtt 
653 0 0 |a fosfoglyceraat kinase (gtp) 
653 0 0 |a phosphoglycerate kinase (gtp) 
653 0 0 |a enzymactiviteit 
653 0 0 |a enzyme activity 
653 0 0 |a biochemische omzettingen 
653 0 0 |a biochemical pathways 
653 1 0 |a Proteins and Enzymes 
653 1 0 |a Eiwitten en enzymen 
700 1 |a Balch, William Edward,  |d 1949-  |e editor. 
700 1 |a Der, Channing J.,  |e editor. 
700 1 |a Hall, A.  |q (Alan),  |e editor. 
776 0 8 |i Print version:  |t Regulators and effectors of small GTPases.  |d San Diego, California : Elsevier Academic Press, �2006  |z 0121828115  |w (OCoLC)63680740 
830 0 |a Methods in enzymology ;  |v v. 406.  |x 0076-6879 
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856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/406  |z Texto completo 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879  |z Texto completo