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G protein pathways. Volume 344, Part B, G proteins and their regulators /
This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Metho...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier,
2001.
|
| Colección: | Methods in enzymology ;
v. 344. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 343; Preface; Volume in Series; Section I: G Protein-Coupled Receptors; A. Theoretical Evaluation of Receptor Function; Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupled Receptors; Chapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of Three-State Model; B. Design and Use of Receptor Ligands; Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function.
- Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled ReceptorsChapter 5. Design of Peptide Agonists; Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors; Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A Receptor; C. Structural Characterization of Receptor Proteins; Chapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function of G Protein-Coupled Receptors; Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors.
- Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavage MethodsChapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the �2-Adrenoceptor; Chapter 12. Crystallization of Membrane Proteins in Cubo; Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and Determining Functional Roles; Chapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin; Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin.
- D. Design and Use of Engineered Receptor ProteinsChapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic Ligands; Chapter 17. Analysis of Structure-Function from Expression of G Protein-Coupled Receptor Fragments; Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor-G Protein Fusion Proteins; Chapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure Function Studies of Receptors; Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors.
- E. Molecular Modeling Studies of Receptor Structure and FunctionChapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled Receptors by Geometric Complementarity Using the GRAMM Computer Algorithm; Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanisms; F. Analysis of Receptor Protein Coupling; Chapter 23. Reconstitution of G Protein-Coupled Receptors with Recombinant GProteinaand �? Subunits; Chapter 24. Cell-Free Membrane Desensitization Assay for G Protein-Coupled Receptors.