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Adult stem cells /
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on e...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California :
Elsevier Academic Press,
�2006.
|
| Colección: | Methods in enzymology ;
v. 419. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Ectoderm
- Chapter 1: Neural Stem cell isolation and characterization
- Reagents and Instrumentation
- Methods
- Chapter 2: Neural stem cells and their manipulation
- Adult niches for stem cells In vivo
- In vitro manipulation of NSCs
- Sample protocols for the culture and characterization of NSCs
- Chapter 3: Retinal stem cells
- Materials and methods
- Chapter 4: Epithelial skin stem cells
- Introduction to skin epithelial stem cells
- Epithelial skin organization and the hair cycle
- Characteristics of skin epithelial stem cells
- Chapter 5: Dental pulp stem cells
- Identification of dental pulp stem cells
- Isolation of DPSCs
- Differentiation of DPSCs
- Section II: Mesoderm
- Chapter 6: Postnatal skeletal stem cells
- Introduction
- Skeletal stem cells and mesenchymal stem cells
- Defining a skeletal stem cell
- Origin and nature of skeletal stem cells
- Plasticity of BMSCs
- Role of skeletal stem cells in disease
- Molecular engineering
- Potential use of skeletal stem cells in tissue engineering and regenerative Medicine
- Establishment of nonclonal populations of BMSCs in vitro
- Establishment of clonal populations of BMSCs in vitro
- Determination of colony-forming efficiency
- In vitro differentiation of BMSCs
- In vivo differentiation of BMSCs
- Chapter 7: Hematopoietic stem Cells
- Identification and enrichment of HSCs
- Functional characterization of candidate HSCs
- Long-term culture of candidate HSCs and progenitors
- Chapter 8: Hemangioblasts and their progeny
- Ex vivo expansion of CD133+ cells
- Hematopoietic and endothelial differentiation of single cell-derived EGFP+ clones
- Phenotypical and functional studies of CD133-derived cells during ex vivo expansion and differentiation
- Materials
- Reagents
- Methods
- Chapter 9: Kidney epithelial cells
- Analysis of steady state tubular epithelium maintenance
- Regeneration of experimentally injured kidney tubules
- Extratubular progenitor cells
- Culture of human tubular progenitor cells
- Chapter 10: Ovarian germ cells
- Origin of primordial germ cells
- Formation of human fetal oocytes and primordial and primary follicles
- Immune system-related cells and oogenesis in fetal ovaries
- Fetal programming of follicular renewal during adulthood
- "Ovary within the ovary " pattern and Thy-1 differentiation protein
- Cessation of adult oogenesis
- Oogenesis and follicular renewal in adult ovaries
- Working hypothesis on the role of the gonadal environment
- Differentiation of stem cells and tissue control system theory
- Potential treatment of ovarian infertility by producing new primary follicles In vivo
- Mystery of the origin of oocytes in adult mammalian ovaries.