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Autophagy in mammalian systems. Part B /

Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house-cleaning" in the cell is called autophagy - literally self-eating. Breakthroughs in unde...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Klionsky, Daniel J. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego, California ; London : Academic Press/Elsevier, �2009.
Colección:Methods in enzymology ; volume 452.
Temas:
Acceso en línea:Texto completo
Texto completo
Texto completo

MARC

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245 0 0 |a Autophagy in mammalian systems.  |n Part B /  |c edited by Daniel J. Klionsky. 
246 3 |a Autophagy 
260 |a San Diego, California ;  |a London :  |b Academic Press/Elsevier,  |c �2009. 
300 |a 1 online resource (xlix, 508 pages, 12 unnumbered pages of plates) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v v. 452 
500 |a Called Part B in continuation of Part A issued as volume 451 of Methods in enzymology under title: Autophagy : Lower eukaryotes and non-mammalian species, Part A. 
504 |a Includes bibliographical references and indexes. 
505 0 |a Monitoring autophagy in mammalian cultured cells through the dynamics of LC3 / Shunsuke Kimura [and others] -- Methods for monitoring autophagy Using GFP-LC3 transgenic mice / Noboru Mizushima -- Using photoactivatable proteins to monitor autophagosome lifetime / Dale W. Hailey and Jennifer Lippincott-Schwartz -- Assaying of autophagic protein degradation / Chantal Bauvy, Alfred J. Meijer, and Patrice Codogno -- Sequestration assays for mammalian autophagy / Per O. Seglen, Anders �verbye, and Frank S�tre -- Assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using Monodansylcadaverine (MDC) and DQ-BSA / Cristina Lourdes V�azquez and Mar�ia Isabel Colombo -- The GST-BHMT assay and related assays for autophagy / Patrick B. Dennis and Carol A. Mercer -- Monitoring starvation-induced reactive oxygen species formation / Ruth Scherz-Shouval and Zvulun Elazar -- Flow cytometric analysis of autophagy in living mammalian cells / Elena Shvets and Zvulun Elazar -- Monitoring autophagy by electron microscopy in mammalian cells / P�aivi Yl�a-Anttila [and others] -- Monitoring mammalian target of Rapamycin (mTOR) activity / Tsuneo Ikenoue, Sungki Hong, and Ken Inoki -- Monitoring autophagic degradation of p62/SQSTM1 / Geir Bj�rk�y [and others] -- Cytosolic LC3 ratio as a quantitative index of macroautophagy / Motoni Kadowaki and Md. Razaul Karim -- Method for monitoring pexophagy in mammalian cells / Junji Ezaki [and others] -- Mitophagy in mammalian cells : the reticulocyte model / Ji Zhang, Mondira Kundu, and Paul A. Ney -- Assessing mammalian autophagy by WIPI-1/Atg18 Puncta formation / Tassula Proikas-Cezanne and Simon G. Pfisterer-- Correlative light and electron microscopy / Minoo Razi and Sharon A. Tooze -- Semiconductor nanocrystals in autophagy research : methodology improvement at nanosized scale / Oleksandr Seleverstov, James M. Phang, and Olga Zabirnyk -- Methods to monitor Chaperone-mediated autophagy / Susmita Kaushik and Ana Maria Cuervo -- Methods to monitor autophagy of Salmonella enterica serovar Typhimurium / Cheryl L. Birmingham and John H. Brumell -- Monitoring autophagy during mycobacterium tuberculosis infection / Marisa Ponpuak [and others] -- Streptococcus-, Shigella-, and Listeria-induced autophagy / Michinaga Ogawa [and others] -- Kinetic analysis of autophagosome formation and turnover in primary mouse macrophages / Michele S. Swanson, Brenda G. Byrne, and Jean-Francois Dubuisson -- Monitoring macroautophagy by major histocompatibility complex class II presentation of targeted antigens / Monique Gannag�e and Christian M�unz -- Detachment-induced autophagy in three-dimensional epithelial cell cultures / Jayanta Debnath -- Methods for inducing and monitoring liver autophagy relative to aging and antiaging caloric restriction in rats / Alessio Donati, Gabriella Cavallini, and Ettore Bergamini -- Physiological autophagy in the Syrian hamster Harderian gland / Ignacio Vega-Naredo and Ana Coto-Montes. 
588 0 |a Print version record. 
520 |a Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house-cleaning" in the cell is called autophagy - literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 in yeast. These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth. In this volume, a robust slate of methods for conducting research are presented, establishing a consensus of appropriate criteria for monitoring autophagy. Along with Autophagy: Lower Eukaryotes, this volume marks the seminal collection of methods in the burgeoning field of autophagy 
650 0 |a Cellular control mechanisms. 
650 0 |a Autophagic vacuoles. 
650 0 |a Apoptosis. 
650 0 |a Eukaryotic cells. 
650 0 |a Biological assay. 
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650 1 2 |a Cells  |x metabolism  |0 (DNLM)D002477Q000378 
650 2 |a Apoptosis  |0 (DNLM)D017209 
650 2 |a Eukaryotic Cells  |0 (DNLM)D005057 
650 2 2 |a Mammals  |x metabolism  |0 (DNLM)D008322Q000378 
650 2 2 |a Microtubule-Associated Proteins  |x metabolism  |0 (DNLM)D008869Q000378 
650 2 2 |a Biological Assay  |0 (DNLM)D001681 
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650 6 |a Apoptose.  |0 (CaQQLa)201-0222225 
650 6 |a Cellules eucaryotes.  |0 (CaQQLa)201-0041437 
650 6 |a Essais biologiques.  |0 (CaQQLa)201-0014647 
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653 0 0 |a degradatie 
653 0 0 |a degradation 
653 0 0 |a katabolisme 
653 0 0 |a catabolism 
653 0 0 |a celfysiologie 
653 0 0 |a cell physiology 
653 1 0 |a Metabolic Processes 
653 1 0 |a Metabole processen 
700 1 |a Klionsky, Daniel J.,  |e editor. 
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