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RNA turnover in eukaryotes : Nucleases, pathways and analysis of mRNA decay /
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California ; London :
Academic Press/Elsevier,
�2008.
|
| Colección: | Methods in enzymology ;
v. 448. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Analysis of mRNA decapping / S.W. Liu [and others]
- A kinetic assay to monitor RNA decapping under single- turnover conditions / B.N. Jones [and others]
- Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / S. Tharun
- Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / B.L. Zaric and C. Kambach
- Regulated deadenylation in vitro / A.C. Goldstrohm, B.A. Hook and M. Wickens
- Cell-free deadenylation assays with Drosophila embryo extracts / M. Jeske and E. Wahle
- Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / J.H. Kim and J.D. Richter
- The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / K.J. Sokoloski, J. Wilusz and C.J. Wilusz
- In vitro assays of 5' to 3'-exoribonuclease activity / O. Pellegrini [and others]
- Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays / J.C. Greimann and C.D. Lima
- Biochemical studies of the mammalian exosome with intact cells / G. Schilders and G.J. Pruijn
- Determining in vivo activity of the yeast cytoplasmic exosome / D. Schaeffer [and others]
- Approaches for studying PMR1 endonuclease-mediated mRNA decay / Y. Otsuka and D.R. Schoenberg
- Methods to determine mRNA half-life in Saccharomyces cerevisiae / J. Coller
- mRNA Decay analysis in Drosophila melanogaster drug-induced changes in glutathione S-transferase D21 mRNA stability / B. Akgul and C.P. Tu
- Measuring mRNA stability during early Drosophila embryogenesis / J.L. Semotok [and others]
- Messenger RNA half-life measurements in mammalian cells / C.Y. Chen, N. Ezzeddine and A.B. Shyu
- Trypanosomes as a model to investigate mRNA decay pathways / S. Archer [and others]
- Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil / M.D. Cleary
- Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae / D.O. Passos and R. Parker
- Transcriptome targets of the exosome complex in plants / D. Belostotsky
- Sensitive detection of mRNA decay products by use of reverse-ligation-mediated PCR (RL-PCR) / T. Grange
- Tethering assays to investigate nonsense-mediated mRNA decay activating proteins / N.H. Gehring, M.W. Hentze and A.E. Kulozik
- Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells / E.L. Murray and D.R. Schoenberg
- Analyzing P-bodies in Saccharomyces cerevisiae / T. Nissan and R. Parker
- Real-time and quantitative imaging of mammalian stress granules and processing bodies / N. Kedersha [and others]
- Cell biology of mRNA decay / D. Grunwald, R.H. Singer and K. Czaplinski.