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Heterotrimeric G proteins /
This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques appli...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in enzymology ;
v. 237. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
MARC
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| 008 | 950202s1994 caua ob 001 0 eng d | ||
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| 042 | |a dlr | ||
| 050 | 4 | |a QP601 |b .M94 vol. 237 | |
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| 060 | 4 | |a QU 55 |b H589 1994 | |
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| 245 | 0 | 0 | |a Heterotrimeric G proteins / |c edited by Ravi Iyengar. |
| 260 | |a San Diego : |b Academic Press, |c �1994. | ||
| 300 | |a 1 online resource (xxx, 561 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 237 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered. | ||
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2011. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2011 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 546 | |a English. | ||
| 505 | 0 | |6 880-01 |a Measurement of receptor-stimulated guanosine 5�a#x80;²-O-(�I³-thio)triphosphate binding by G proteins / Thomas Wieland, Karl H Jakobs -- Receptor-stimulated hydrolysis of guanosine 5�a#x80;²-triphosphate in membrane preparations / Peter Gierschik, Thomas Bouillon, Karl H Jakobs -- Regulation of G-protein activation by mastoparans and other cationic peptides / Elliott M Ross, Tsutomu Higashuima -- Guanosine 5�a#x80;²-O-(�I³-thio)triphosphate binding assay for solubilized G proteins / Donna J Carty, Ravi Iyengar -- Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins / Joel Moss, Randy S Haun, Su-Chen Tsai, Catherine F Welsh, Fang-Jen Scott Lee, S Russ Price, Martha Vaughan -- Pertussis toxin-catalyzed ADP-ribosylation of G proteins / Donna J Carty -- Synthesis and use of radioactive photoactivatable NAD+ derivatives as probes for G-protein structure / Richard R Vaillancourt, N Dhanasekaran, Arnold E Ruoho -- Photoaffinity guanosine 5�a#x80;²-triphosphate analogs as a tool for the study of GTP-binding proteins / Mark M Rasenick, Madhavi Talluri, William J Dunn III -- Preparation of activated �I� subunits of Gs and GiS: From erythrocyte to activated subunit / Lutz Birnbaumer, Dagoberto Grenet, Fernando Ribeiro-Neto, Juan Codina -- Purification and separation of closely related members of pertussis toxin-substrate G proteins / Toshiaki Katada, Kenji Kontani, Atsushi Inanobe, Ichiro Kobayashi, Yoshiharu Ohoka, Hiroshi Nishina, Katsunobu Takahashi -- Purification of transducin / Joe�I#x88;lle Bigay, Marc Chabre -- Expression of G-protein a subunits in Escherichia coli / Ethan Lee, Maurine E Linder, Alfred G Gilman -- Synthesis and applications of affinity matrix containing immobilized �I²�I³ subunits of G proteins / Iok-Hou Pang, Alan V Smrcka, Paul C Sternweis -- Purification of activated and heterotrimeric forms of Gq proteins / Jonathan L Blank, John H Exton -- Purification of Phospholipase C-activating G protein, G11, from Turkey erythrocytes / Gary L Waldo, Jose�I#x81; L Boyer, T Kendall Harden -- Purification of recombinant Gq�I�, G11�I�, and G16�I� from Sf9 cells / John R Hepler, Tohru Kozasa, Alfred G Gilman -- Expression and purification of G-protein �I� subunits using baculovirus expression system / Stephen G Graber, Robert A Figler, James C Garrison -- Analysis of G-protein �I� and �I²�I³ subunits by in vitro translation / Eva J Neer, Bradley M Denker, Thomas C Thomas, Carl J Schmidt -- Assays for studying functional properties of in vitro translated Gs�I� subunit / Yves Audigier -- Myristoylation of G-protein �I� subunits / Susanne M Mumby, Maurine E Linder -- Specificity and functional applications of antipeptide antisera which identify G-protein �I� subunits / Graeme Milligan -- Identification of receptor-activated G proteins: Selective immunoprecipitation of photolabeled G-protein �I� subunits / Karl-Ludwig Laugwitz, Karsten Spicher, Gu�I#x88;nter Schultz, Stefan Offermanns -- Identification of mutant forms of G-protein �I� subunits in human neoplasia by polymerase chain reaction-based techniques / John Lyons -- Detection of mutations and polymorphisms of Gs�I� subunit gene by denaturing gradient Gel electrophoresis / Pablo V Gejman, Lee S Weinstein -- Construction of mutant and chimeric G-protein �I� subunits / Sim Winitz, Marijane Russell, Gary L Johnson -- Design of degenerate oligonucleotide primers for cloning of G-protein a subunits / Thomas M Wilkie, Anna M Aragay, A John Watson, Melvin I Simon -- Microinjection of antisense oligonucleotides to assess G-protein subunit function / Christiane Kleuss, Gu�I#x88;nter Schultz, Burghardt Wittig -- Inactivation of G-protein genes: Double knockout in cell lines / Richard M Mortensen, J.G Seidman -- Targeted Inactivation of the G12�I� gene with replacement and insertion vectors: Analysis in a 96-well plate format / Uwe Rudolph, Allan Bradley, Lutz Birnbaumer -- G-Protein assays in Dictyostelium / B Ewa Snaar-Jagalska, Peter J.M Van Haastert -- Fluorescence assays for G-protein interactions / Richard A Cerione -- Specific peptide probes for G-protein interactions with receptors / Heidi E Hamm, Helen M Rarick -- Vaccinia virus systems for expression of G�I� genes in S49 cells / Franklin Quan, Michael Forte -- Purification of T�I²�I³ subunit of transducin / Joe�I#x88;lle Bigay, Marc Chabre -- Adenylyl cyclase assay for �I²�I³ subunits of G proteins / Jianqiang Chen, Donna J Carty, Ravi Iyengar -- Synthesis and use of biotinylated �I²�I³ complexes prepared from bovine brain G proteins / Jane Dingus, Michael D Wilcox, Russell Kohnken, John D Hildebrandt -- Design of oligonucleotide probes for molecular cloning of �I² and �I³ subunits / Christine Gallagher, Narasimhan Gautam -- Characterization of antibodies for various G-protein �I² and �I³ subunits / Alexey N Pronin, Narasimhan Gautam -- Preparation, characterization, and use of antibodies with specificity for G-protein y subunits / Janet D Robishaw, Eric A Balcueva -- Isoprenylation of �I³ subunits and G-protein effectors / Bernard K.-K Fung, Janmeet S Anant, Wun-Chen Lin, Olivia C Ong, Harvey K Yamane. | |
| 650 | 0 | |a G proteins. | |
| 650 | 1 | 2 | |a GTP-Binding Proteins |x analysis |0 (DNLM)D019204Q000032 |
| 650 | 2 | |a GTP-Binding Proteins |0 (DNLM)D019204 | |
| 650 | 6 | |a Prot�eines G. |0 (CaQQLa)201-0203891 | |
| 650 | 7 | |a G proteins. |2 fast |0 (OCoLC)fst00936830 | |
| 650 | 7 | |a Prot�eines liant GTP. |2 fmesh | |
| 650 | 7 | |a Transduction du signal. |2 fmesh | |
| 650 | 7 | |a GTP-bindende Proteine |2 gnd |0 (DE-588)4248354-2 | |
| 650 | 7 | |a Aufsatzsammlung |2 gnd |0 (DE-588)4143413-4 | |
| 650 | 1 | 7 | |a Prote�ine-G. |2 gtt |
| 650 | 1 | 7 | |a Biochemie. |2 gtt |
| 650 | 7 | |a Prot�eines G. |2 ram | |
| 650 | 7 | |a prot�eine G |v �etudes diverses. |2 rero | |
| 653 | 0 | |a Proteins | |
| 700 | 1 | |a Iyengar, Ravi, |e edtor. | |
| 776 | 0 | 8 | |i Print version: |t Heterotrimeric G proteins. |d San Diego : Academic Press, �1994 |z 0121821382 |w (OCoLC)30636280 |
| 830 | 0 | |a Methods in enzymology ; |v v. 237. |x 0076-6879 | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780121821388 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/237 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879 |z Texto completo |
| 880 | 0 | 0 | |6 505-01/(S |t Measurement of receptor-stimulated guanosine 52-O-(γ-thio)triphosphate binding by G proteins / |r Thomas Wieland, Karl H. Jakobs -- |t Receptor-stimulated hydrolysis of guanosine 52-triphosphate in membrane preparations / |r Peter Gierschik, Thomas Bouillon, Karl H. Jakobs -- |t Regulation of G-protein activation by mastoparans and other cationic peptides / |r Elliott M Ross, Tsutomu Higashuima -- |t Guanosine 52-O-(γ-thio)triphosphate binding assay for solubilized G proteins / |r Donna J. Carty, Ravi Iyengar -- |t Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins / |r Joel Moss, Randy S Haun, Su-Chen Tsai, Catherine F Welsh, Fang-Jen Scott Lee, S Russ Price, Martha Vaughan -- |t Pertussis toxin-catalyzed ADP-ribosylation of G proteins / |r Donna J. Carty -- |t Synthesis and use of radioactive photoactivatable NAD+ derivatives as probes for G-protein structure / |r Richard R. Vaillancourt, N. Dhanasekaran, Arnold E. Ruoho -- |t Photoaffinity guanosine 52-triphosphate analogs as a tool for the study of GTP-binding proteins / |r Mark M. Rasenick, Madhavi Talluri, William J. Dunn III -- |t Preparation of activated α subunits of Gs and GiS: From erythrocyte to activated subunit / |r Lutz Birnbaumer, Dagoberto Grenet, Fernando Ribeiro-Neto, Juan Codina -- |t Purification and separation of closely related members of pertussis toxin-substrate G proteins / |r Toshiaki Katada, Kenji Kontani, Atsushi Inanobe, Ichiro Kobayashi, Yoshiharu Ohoka, Hiroshi Nishina, Katsunobu Takahashi -- |t Purification of transducin / |r Jo�elle Bigay, Marc Chabre -- |t Expression of G-protein a subunits in Escherichia coli / |r Ethan Lee, Maurine E. Linder, Alfred G. Gilman -- |t Synthesis and applications of affinity matrix containing immobilized βγ subunits of G proteins / |r Iok-Hou Pang, Alan V. Smrcka, Paul C. Sternweis -- |t Purification of activated and heterotrimeric forms of Gq proteins / |r Jonathan L. Blank, John H. Exton -- |t Purification of Phospholipase C-activating G protein, G11, from Turkey erythrocytes / |r Gary L. Waldo, Jos�e L. Boyer, T. Kendall Harden -- |t Purification of recombinant Gqα, G11α, and G16α from Sf9 cells / |r John R. Hepler, Tohru Kozasa, Alfred G. Gilman -- |t Expression and purification of G-protein α subunits using baculovirus expression system / |r Stephen G. Graber, Robert A. Figler, James C. Garrison -- |t Analysis of G-protein α and βγ subunits by in vitro translation / |r Eva J. Neer, Bradley M. Denker, Thomas C. Thomas, Carl J. Schmidt -- |t Assays for studying functional properties of in vitro translated Gsα subunit / |r Yves Audigier -- |t Myristoylation of G-protein α subunits / |r Susanne M. Mumby, Maurine E. Linder -- |t Specificity and functional applications of antipeptide antisera which identify G-protein α subunits / |r Graeme Milligan -- |t Identification of receptor-activated G proteins: Selective immunoprecipitation of photolabeled G-protein α subunits / |r Karl-Ludwig Laugwitz, Karsten Spicher, G�unter Schultz, Stefan Offermanns -- |t Identification of mutant forms of G-protein α subunits in human neoplasia by polymerase chain reaction-based techniques / |r John Lyons -- |t Detection of mutations and polymorphisms of Gsα subunit gene by denaturing gradient Gel electrophoresis / |r Pablo V. Gejman, Lee S. Weinstein -- |t Construction of mutant and chimeric G-protein α subunits / |r Sim Winitz, Marijane Russell, Gary L. Johnson -- |t Design of degenerate oligonucleotide primers for cloning of G-protein a subunits / |r Thomas M. Wilkie, Anna M. Aragay, A. John Watson, Melvin I. Simon -- |t Microinjection of antisense oligonucleotides to assess G-protein subunit function / |r Christiane Kleuss, G�unter Schultz, Burghardt Wittig -- |t Inactivation of G-protein genes: Double knockout in cell lines / |r Richard M. Mortensen, J.G. Seidman -- |t Targeted Inactivation of the G12α gene with replacement and insertion vectors: Analysis in a 96-well plate format / |r Uwe Rudolph, Allan Bradley, Lutz Birnbaumer -- |t G-Protein assays in Dictyostelium / |r B. Ewa Snaar-Jagalska, Peter J.M. Van Haastert -- |t Fluorescence assays for G-protein interactions / |r Richard A. Cerione -- |t Specific peptide probes for G-protein interactions with receptors / |r Heidi E. Hamm, Helen M. Rarick -- |t Vaccinia virus systems for expression of Gα genes in S49 cells / |r Franklin Quan, Michael Forte -- |t Purification of Tβγ subunit of transducin / |r Jo�elle Bigay, Marc Chabre -- |t Adenylyl cyclase assay for βγ subunits of G proteins / |r Jianqiang Chen, Donna J. Carty, Ravi Iyengar -- |t Synthesis and use of biotinylated βγ complexes prepared from bovine brain G proteins / |r Jane Dingus, Michael D. Wilcox, Russell Kohnken, John D. Hildebrandt -- |t Design of oligonucleotide probes for molecular cloning of β and γ subunits / |r Christine Gallagher, Narasimhan Gautam -- |t Characterization of antibodies for various G-protein β and γ subunits / |r Alexey N. Pronin, Narasimhan Gautam -- |t Preparation, characterization, and use of antibodies with specificity for G-protein y subunits / |r Janet D. Robishaw, Eric A. Balcueva -- |t Isoprenylation of γ subunits and G-protein effectors / |r Bernard K.-K Fung, Janmeet S. Anant, Wun-Chen Lin, Olivia C. Ong, Harvey K. Yamane. |