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Cell culture /

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much materia...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Jakoby, William B., 1928- (Editor ), Pastan, Ira H. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1979.
Colección:Methods in enzymology ; v. 58.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Section I. Basic methods
  • A. Laboratory requirements and media
  • B. General cell culture techniques
  • Section II. Specialized techniques
  • A. Metabolism
  • B. Genetics, hybridization, and transformation
  • C. Virus preparation
  • Section III. Specific cell lines.
  • Physical aspects of a tissue culture laboratory / William H.J. Douglas, Robert T. Dell'Orco
  • Detection of contamination / Gerard J. McGarrity
  • Preservation, storage, and shipment / Lewis L. Coriell
  • Safety considerations in the cell culture laboratory / W. Emmett Barkley
  • Media and growth requirements / Richard G. Ham, Wallace L. McKeehan
  • The growth of cells in serum-free hormone-supplemented media / Jane Bottenstein, Izumi Hayashi, Sharon Hutchings, Hideo Masui, Jennie Mather, Don B. McClure, Sugayuki Ohasa, Angie Rizzino, Gordon Sato, Ginette Serrero, Richard Wolfe, Reen Wu
  • Use of antibiotics in cell culture media / D. Perlman
  • Laboratory management of cell cultures / Ken Wolf
  • Dispersion and disruption of tissues / Mark M. Bashor
  • Monolayer culture techniques / James A. McAteer, William H.J. Douglas
  • Measurement of growth and viability of cells in culture / Manford K. Patterson Jr.
  • Cloning / Lola C.M. Reid
  • Cell culture characterization: Monitoring for cell identification / Ward D. Peterson Jr., William F. Simpson, Bharati Hukku
  • Tissue Culture on Artificial Capillaries / P.M. Gullino, R.A. Knazek
  • Microcarrier culture: A homogeneous environment for studies of cellular biochemistry / William G. Thilly, David W. Levine
  • Mass culture of mammalian cells / William F. McLimans
  • Propagation and scaling-up of suspension cultures / Ronald T. Acton, Paul A. Barstad, Robert K. Zwerner
  • Harvesting the products of cell growth / Robert K. Zwerner, Kim S. Wise, Ronald T. Action
  • Cell cycle analysis by flow cytometry / J.W. Gray, P. Coffino
  • Cell synchronization / Tsukasa Ashihara, Renato Baserga
  • New Techniques for Culturing Differential Cells: Reconstituted basement membrane rafts / Lola M. Reid, Marcos Rojkind
  • Autoradiography / Gretchen H. Stein, Rosalind Yanishevsky
  • Induction and production of interferon / Robert M. Friedman
  • Evaluation of chemical carcinogenicity by in vitro neoplastic transformation / No�el Bouck, Giampiero di Mayorca
  • Independent control of the local environment of somas and neurites / Robert B. Campenot
  • Mutant isolation / Larry H. Thompson
  • Karyotyping / Ronald G. Worton, Catherine Duff
  • Cell fusion / Roger H. Kennett
  • Fusion of higher plant protoplasts / Albert W. Ruesink
  • Cell transformation / Ira Pastan
  • Use of nude mice for tumorigenicity testing and mass propagation / Seung-il Shin
  • Biological techniques for avian sarcoma viruses / Eric Hunter
  • Large-scale growth of Rous sarcoma virus / Ralph E. Smith
  • Preparation of Simian Virus 40 and its DNA / George Khoury, Ching-Ju Lai
  • Purification and assay of murine leukemia viruses / Charles J. Sherr, George J. Todaro
  • Human adenoviruses: Growth, purification, and transfection assay / Maurice Green, William S.M. Wold
  • Sources of stable cell lines / Gerard J. McGarrity
  • Isolation of fibroblasts from patients / William S. Sly, Jeffrey Grubb
  • Cell lines from invertebrates / W. Fred Hink
  • Cold-blooded vertebrate cell and tissue culture / Ken Wolf
  • Plant cell lines / John F. Reynolds, Toshio Murashige
  • Lymphocytes as resting cells / Shelby L. Berger
  • Macrophages / Dolph O. Adams
  • Mouse erythroleukemia cells / T.V. Gopalakrishnan, W. French Anderson
  • Skeletal myoblasts in culture / Irwin R. Konigsberg
  • Clonal strains of hormone-producing pituitary cells / Armen H. Tashjian Jr.
  • Liver cells / H.L. Leffert, K.S. Koch, T. Moran, M. Williams
  • Liver cells (HTC) / E. Brad Thompson
  • An established but differentiated kidney epithelial cell line (MDCK) / Mary Taub, Milton H. Saier Jr.
  • Large-scale preparation of chondrocytes / Michael Klagsbrun
  • Melanoma cells / John Pawelek
  • Adrenocortical Y1 cells / Bernard P. Schimmer
  • Long-term culture of dissociated sympathetic neurons / Edward Hawrot, Paul H. Patterson
  • Neuronal cells from rodent neoplasma / David Schubert, William Carlisle.