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Guide to protein purification /
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features *...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1990.
|
| Colección: | Methods in enzymology ;
v. 182. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Guide to protein purification / |c edited by Murray P. Deutscher. |
| 260 | |a San Diego : |b Academic Press, |c �1990. | ||
| 300 | |a 1 online resource (xxix, 894 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 182 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure. | ||
| 505 | 0 | |a Why purify enzymes / Arthur Kornberg -- Strategies and considerations for protein purifications / Stuart Linn -- Setting up a laboratory / Murray P. Deutscher -- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard -- Measurement of enzyme activity / Edward F. Rossomando -- Quantitation of protein / Christa M. Stoscheck -- Concentration of proteins and removal of solutes / Thomas Pohl -- Maintaining protein stability / Murray P. Deutscher -- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das -- Overexpression of proteins in eukaryotes / Margaret K. Bradley -- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Pl�uckthun -- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry -- Preparation of extracts from yeast / S. Michal Jazwinski -- Preparation of Extracts from Plants / Peter Gegenheimer -- Preparation of extracts from higher eukaryotes / John David Dignam -- Isolation of subcellular organelles / Brian Storrie, Edward A Madden -- Preparation of membrane fractions / Juris Ozols -- Detergents: An overview / Judith M. Neugebauer -- Solubilization of native membrane proteins / Leonard M. Hjelmeland -- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley -- Removal of detergents from membrane proteins / Leonard M. Hjelmeland -- Precipitation techniques / Sasha Englard, Sam Seifter -- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham -- Ion-exchange chromatography / Edward F. Rossomando -- Gel filtration / Earle Stellwagen -- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff -- Hydrophobic chromatography / Robert M. Kennedy -- Chromatography on immobilized reactive dyes / Earle Stellwagen -- Affinity chromatography: General methods / Steven Ostrove -- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier -- One-dimensional gel electrophoresis / David E. Garfin -- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons -- Isoelectric focusing / David E. Garfin -- Gel-staining techniques / Carl R. Merril -- Elution of protein from gels / Michael G. Harrington -- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee -- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker -- Purification of glycoproteins / Craig Gerard -- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews -- Determination of purity / David G. Rhodes, Thomas M. Laue -- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes -- Amino acid analysis / Juris Ozols -- Limited N-terminal sequence analysis / Paul Matsudaira -- Peptide mapping / Ralph C. Judd -- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard -- Protein crystallization / David Ollis, Stephen White -- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel -- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner -- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar -- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth -- Immunoassays / Charles W. Parker -- Radiolabeling of proteins / Charles W. Parker -- Using purified protein to clone its gene / John M. Wozney -- Computer analysis of protein structure / Patrick Argos -- Rethinking your purification procedure / Murray P. Deutscher -- The private life of DNA polymerase I / Arthur Kornberg -- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas -- Discovery of glutamine synthetase cascade / Earl R. Stadtman -- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2011. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2011 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 546 | |a English. | ||
| 650 | 0 | |a Proteins |x Purification. | |
| 650 | 2 | |a Proteins |x isolation & purification |0 (DNLM)D011506Q000302 | |
| 650 | 6 | |a Prot�eines |x Purification. |0 (CaQQLa)201-0232417 | |
| 650 | 7 | |a Proteins |x Purification |2 fast |0 (OCoLC)fst01079750 | |
| 650 | 7 | |a Proteine |2 gnd |0 (DE-588)4076388-2 | |
| 650 | 7 | |a Reinigung |2 gnd |0 (DE-588)4049250-3 | |
| 650 | 7 | |a Reinigungsverfahren |2 gnd |0 (DE-588)4391913-3 | |
| 650 | 1 | 7 | |a Eiwitten. |2 gtt |
| 650 | 1 | 7 | |a Enzymen. |2 gtt |
| 650 | 1 | 7 | |a Zuiveringstechnieken. |2 gtt |
| 650 | 1 | 7 | |a Laboratoriumonderzoek. |2 gtt |
| 653 | 0 | |a Proteine |a Reinigungsverfahren | |
| 653 | 0 | |a Proteine |a Reinigung | |
| 700 | 1 | |a Deutscher, Murray P., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Guide to protein purification. |d San Diego : Academic Press, �1990 |z 0121820831 |w (OCoLC)22543482 |
| 830 | 0 | |a Methods in enzymology ; |v v. 182. |x 0076-6879 | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780121820831 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/182 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879 |z Texto completo |