Aqueous two-phase systems /

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Walter, Harry (Editor ), Johansson, G�ote (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1994.
Colección:Methods in enzymology ; v. 228.
Temas:
Separation (Technology)
Phase partition.
Biochemistry > Technique.
Biotechnology > Technique.
Cell Fractionation > methods
Cell Extracts > isolation & purification
S�eparation (Technologie)
Partage des phases.
Biochimie > Technique.
Biotechnologie > Technique.
Biochemistry > Technique
Biotechnology > Technique
Phase partition
Polymers > Biotechnology
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Section I. General methodology and apparatus
  • Section II. Partitioning and affinity partitioning of macromolecules
  • A. Proteins
  • B. Nucleic acids
  • Section III. Studies on protein interactions, molecular structure, charge, hydrophobicity, and conformational changes
  • Section IV. Partitioning and affinity partitioning of particulates
  • A. Cells: Separation and fractionation of cell populations and obtaining information on their surface properties
  • Affinity partitioning
  • B. Organelles: Separation and subfractionation
  • C. Membranes: Separation and subfractionation
  • Affinity partitioning
  • Membrane domain analysis
  • Section V. Aqueous phase separations in biological systems
  • Section VI. Aqueous two-phase systems in large-scale processes: Biotechnology
  • A. Extractive bioconversions
  • B. Proteins: Downstream processing
  • C. Design of proteins for enhanced extraction
  • D. Other applications of aqueous phases in biotechnology.

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