FRET and FLIM techniques /

This volume reviews the techniques F�orster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Gadella, T. W. J. (Theodorus W. J.) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; London : Elsevier, �2009.
Colección:Laboratory techniques in biochemistry and molecular biology ; v. 33.
Temas:
Fluorescence microscopy > Laboratory manuals.
Energy transfer > Laboratory manuals.
Fluorescence Resonance Energy Transfer > methods
Microscopy, Fluorescence > methods
Spectrometry, Fluorescence > methods
Microscopie de fluorescence > Manuels de laboratoire.
Transfert d'�energie > Manuels de laboratoire.
NATURE > Reference.
SCIENCE > Life Sciences > General.
SCIENCE > Life Sciences > Biology.
Energy transfer
Fluorescence microscopy
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • Chapter 1. F�orster Resonance Energy transfer -FRET what is it, why do it, and how it's done / Robert M. Clegg
  • Chapter 2. Frequency domain FLIM theory, Instrumentation and data analysis / Peter J. Verveer and Quentin Hanley
  • Chapter 3. Time Domain FLIM; Theory, Instrumentation / Hans C. Gerritsen, A.V. Agronskaia, A.N. Bader, A. Esposito
  • Chapter 4. Multidimensional fluorescence imaging / James James McGinty, Christopher Dunsby, Egidijus Auksorius, Pieter De Beule, Daniel S. Elson, Neil Galletly, Oliver Hoffman, Gordon Kennedy, Peter M.P. Lanigan, Ian Munro, Bj�orn �Onfelt, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, M. John Lever, Andrew J. deMello, Gordon S. Stamp, Mark A.A. Neil and Paul M.W. French
  • Chapter 5. Visible fluorescent proteins for FRET / Gert-Jan Kremers and Joachim Goedhart
  • Chapter 6. Small Molecule-based FRET probes / Amanda Cobos Correa, Oliver Wichmann and Carsten Schultz
  • Chapter 7. Filter FRET: quantitative imaging of sensitized emission / Kees Jalink and Jacco van Rheenen
  • Chapter 8. Spectral Imaging and its use in the measurement of F�orster Resonance Energy Transfer in living cells / Steven S. Vogel, Paul. S. Blank, Srinagesh V. Koushik and Christopher Thaler
  • Chapter 9. Total Internal Reflection Fluorescence Lifetime Imaging Microscopy / Theodorus W.J. Gadella Jr.
  • Chapter 10. FRET and FLIM applications in plants / Riyaz A. Bhat
  • Chapter 11. Biomedical FRET-FLIM applications / Phill B. Jones, Brian J. Bacskai, Bradley T. Hyman
  • Chapter 12. Reflections on FRET imaging: formalism, probes and implementation / Elizabeth A. Jares-Erijman and Thomas M. Jovin.

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