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Affinity chromatography /
Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; New York : New York :
Elsevier Scientific Pub. Co. ; Distributors for the U.S. and Canada, Elsevier North-Holland,
1978.
|
| Colección: | Journal of chromatography library ;
v. 12. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 100 | 1 | |a Turkov�a, Jaroslava. | |
| 245 | 1 | 0 | |a Affinity chromatography / |c Jaroslava Turkov�a. |
| 260 | |a Amsterdam ; |a New York : |b Elsevier Scientific Pub. Co. ; |a New York : |b Distributors for the U.S. and Canada, Elsevier North-Holland, |c 1978. | ||
| 300 | |a 1 online resource (ix, 405 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Journal of chromatography library ; |v v. 12 | |
| 520 | |a Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances. | ||
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 505 | 0 | |a Front Cover; Affinity Chromatography; Copyright Page; Contents; Acknowledgements; Chapter 1. Introduction; References; Chapter 2. The principle history and use of affinity chromatography; References; Chapter 3. Theory of affiity chromatography; 3.1 Theoretical guidelines deduced on the basis of the equilibrium model; 3.2 Theory of cooperative bonding within the plate theory; 3.3 Statistical theory of chromatography applied to affinity chromatography; References; Chapter 4. Application of affinity chromatography to the quantitative evaluation of specific complexes | |
| 505 | 8 | |a 4.1 Determination of dissociation constants by elution analysis4.2 Determination of dissociation constants by frontal analysis; 4.3 Cooperative elution of oligoadenylic acid in immobilized polyuridylic acid chromatography; 4.4 Other methods for the quantitative evaluation of interactions with immobilized affinity ligands; References; Chapter 5. General considerations on affinant-sorbent bonding; 5.1 Steric accessibility; 5.2 Conformation of attached affinant; 5.3 Concentration of the affinant on the matrix; 5.4 Concentration of proteins equilibration time and flow-rate | |
| 505 | 8 | |a 5.5 Effect of temperature5.6 Effect of pH and ionic strength; 5.7 Elution with competitive affinity ligands; 5.8 Non-specific effects; References; Chapter 6. Choice of affinity ligands for attachment; 6.1 Highly specific and group-specific matrices; 6.2 Isolation of enzymes inhibitors and cofactors; 6.3 Immunoaffinity chromatography; 6.4 Isolation of lectins. glycoproteins and saccharides; 6.5 Isolation of receptors binding and transport protein; 6.6 Isolation of -SH proteins and peptides; 6.7 Isolation of specific peptides; 6.8 Isolation of nucleic acids and nucleotides | |
| 505 | 8 | |a 6.9 Isolation of lipids. hormones and other substances6.10 Isolation of cells and viruses; 6.11 Commercially available insoluble affinants; References; Chapter 7. Hydrophobic chromatography covalent affinity chromatography affinity elution and related methods; 7.1 Hydrophobic chromatography; 7.2 Covalent affinity chromatography; 7.3 Affinity elution; 7.4 Affinity density perturbation; 7.5 Affinity electrophoresis; 7.6 Metal chelate affinity chromatography; References; Chapter 8. Solid matrix supports and the most used methods of binding; 8.1 Required characteristics | |
| 505 | 8 | |a 8.2 Survey of the most common solid supports and coupling procedures8.3 Spacers; 8.4 Blocking of unreacted groups; 8.5 Leakage of the coupled affinant; 8.6 General considerations in the choice of sorbents, spacers and coupling and blocking procedures; References; Chapter 9. Characterization of supports and immobilized affinity ligands; 9.1 Methods for thedetermination of non-specific sorption; 9.2 Determination of activatable and active groups; 9.3 Methods for the determination of immobilized affinity ligands; 9.4 Active-site titration of immobilized proteases | |
| 546 | |a English. | ||
| 650 | 0 | |a Affinity chromatography. | |
| 650 | 2 | |a Chromatography, Affinity |0 (DNLM)D002846 | |
| 650 | 6 | |a Chromatograhie d'affinit�e | |
| 650 | 6 | |a Chromatographie d'affinit�e. |0 (CaQQLa)201-0022808 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Affinity chromatography. |2 fast |0 (OCoLC)fst00798892 | |
| 650 | 7 | |a Chromatographie. |2 fmesh | |
| 776 | 0 | 8 | |i Print version: |a Turkov�a, Jaroslava. |t Affinity chromatography. |d Amsterdam ; New York : Elsevier Scientific Pub. Co. ; New York : Distributors for the U.S. and Canada, Elsevier North-Holland, 1978 |z 0444416056 |z 9780444416056 |w (DLC) 78000815 |w (OCoLC)3627464 |
| 830 | 0 | |a Journal of chromatography library ; |v v. 12. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780444416056 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/03014770/12 |z Texto completo |