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Carbohydrate analysis : high performance liquid chromatography and capillary electrophoresis /
Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; New York :
Elsevier,
1995.
|
| Colección: | Journal of chromatography library ;
v. 58. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; CarbohydrateIm Analysis: High Performance Liquid Chromatography and Capillary Electrophoresis; Copyright Page; Contents; Preface; List of Contributors; Part I: The Solute; Chapter 1. Preparation of Carbohydrates for Analysis by HPLC and HPCE; 1.1 Introduction; 1.2 Sample purification; 1.3 Release of poly- or oligosaccharides from the sample; 1.4 Further degradation for structural study; 1.5 Exchanging solvents; 1.6 Sources for reference compounds; 1.7 References; Part II: Analytical and Preparative Separations
- Chapter 2. Reversed-Phase and Hydrophobic Interaction Chromatography of Carbohydrates and Glycoconjugates2.1 Introduction; 2.2 Fundamentals; 2.3 Stationary phases; 2.4 Mobile phases; 2.5 Separation methodologies and selected applications; 2.5 Acknowledgments; 2.6 References; Chapter 3. High Performance Hydrophilic Interaction Chromatography of Carbohydrates with Polar Sorbents; 3.1 Introduction; 3.2 The chromatographic system; 3.3 Applications of hydrophilic interaction chromatography to carbohydrates; 3.4 Examples of applications of hydrophilic interaction chromatography to carbohy- drates
- 3.5 Conclusions3.6 References; Chapter 4. HPLC of Carbohydrates with Cation- and Anion-Exchange Silica and Resin-Based Stationary Phases; 4.1 Introduction; 4.2 Cation-exchange HPLC of carbohydrates; 4.3 Anion-exchange HPLC of carbohydrates; 4.4 Applications; 4.5 References; Chapter 5. Analysis of Glycoconjugates Using High-pH Anion-Exchange Chromatography; 5.1 Introduction; 5.2 Monosaccharide analysis; 5.3 Oligosaccharide analysis; 5.4 In-line and off-line desalting after HPAEC separations; 5.5 Other important applications; 5.6 Abbreviations; 5.7 References
- Chapter 6. Basic Studies on Carbohydrate
- Protein Interaction by High Performance Affinity Chromatography and High Performance Capillary Affinity Electrophoresis Using Lectins as Protein Models6.1 Introduction; 6.2 Use of high performance liquid chromatography; 6.3 Use of high performance capillary electrophoresis; 6.4 References; Chapter 7. Modern Size-Exclusion Chromatography of Carbohydrates and Glycoconjugates; 7.1 Introduction; 7.2 The chromatographic system; 7.3 Applications of size-exclusion chromatography in the carbohydrate field; 7.4 Conclusions; 7.5 References
- Chapter 8. High Performance Capillary Electrophoresis of Carbohydrates and Glycoconjugates8.1 Introduction; 8.2 Overview of the fundamentals of capillary electrophoresis; 8.3 The electrophoretic system; 8.4 HPCE methodologies and applications; 8.5 Acknowledgments; 8.6 References; Chapter 9. Preparative HPLC of Carbohydrates; 9.1 Introduction; 9.2 Equipment; 9.3 Stationary phases; 9.4 General guidelines for preparative HPLC of carbohydrates; 9.5 Specific preparative HPLC techniques for various classes of carbohydrates; 9.6 Bibliographic information; 9.7 References; Part III: The Detection