The Zebrafish : genetics and genomics /

This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features* Details state-of-the-art zebrafis...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: American Society for Cell Biology
Otros Autores: Detrich, H. William, III, Westerfield, Monte, Zon, Leonard I.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1999.
Colección:Methods in cell biology ; v. 60.
Temas:
Zebra danio > Genetics.
Zebra danios as laboratory animals.
Genomes.
Zebrafish > genetics
Chromosome Mapping > methods
Genome
Mutagenesis
Mutation > genetics
Danio z�ebr�e > G�en�etique.
Danio z�ebr�e (Animal de laboratoire)
G�enomes.
SCIENCE > Life Sciences > Zoology > Ichthyology & Herpetology.
NATURE > Animals > Fish.
Poissons.
Biologie marine.
Biologie mol�eculaire.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Front Cover; Methods in Cell Biology, Volume 60; Copyright Page; Contents; Contributors; Preface; Chapter 1. A Gynogenesis-Based Screen for Maternal-Effect Genes in the Zebrafish, Danio rerio; I. Introduction; II. Optimization of the Gynogenetic Method; III. A Pilot Screen for Maternal-Effect Mutations; IV. Recovery of Mutations; V. Solutions, Materials, and Protocols for Gynogenetic Methods; VI. Conclusions; References; Chapter 2. Developmental Mutant Screens in the Zebrafish; I. Introduction; II. Mutagenesis; III. Breeding Scheme; IV. Screening Procedure; V. Allele Screens
  • VI. Dominant ScreensVII. Managing the Outcome of Screens; VIII. Potential of Future Screens; Appendix-Recipes and Media; References; Chapter 3. Haploid Screens and Gamma-Ray Mutagenesis; I. Introduction; II. Production of Haploid Embryos; III. Development of Haploid Embryos; IV. Genetic Background; V. The Haploid Screen; VI. Limitations of Haploids; VII. Mosaicism in F1 Screens; VIII. Haploid Screens and Gamma-Ray Mutagenesis; IX. Nature of Gamma-Ray-Induced Mutations; X. Conclusion; References; Chapter 4. Early Pressure Screens; I. Introduction
  • II. Rationale for Use of Early Pressure to Produce Diploid Embryos for Genetic ScreensIII. Rationale for Use of Screens Focused on Specific Phenotypes; IV. Design of Our Screen and Mutant Phenotypes Isolated; V. Closing Remarks; References; Chapter 5. Retrovirus-Mediated Insertional Mutagenesis in Zebrafish; I. Introduction; II. Insertional Mutagenesis: A Pilot Screen; III. Considerations for a Large-Scale Screen; References; Chapter 6. Genetic Applications of Transposons and Other Repetitive Elements in Zebrafish; I. Introduction; II. Classification of Repetitive Elements
  • III. Genetic Applications of Repetitive ElementsIV. Summary and Perspectives; References; Chapter 7. Transgenesis; I. Introduction; II. Materials; III. Methods; IV. Discussion; References; Chapter 8. The Zebrafish Genome; I. Introduction; II. Mapping the Zebrafish Genome; III. The Zebrafish Gene Map; IV. Two Genes in Zebrafish for One in Mammals; V. Gene Nomenclature in Zebrafish; VI. Conclusions; References; Chapter 9. Using Random Amplified Polymorphic DNAs in Zebrafish Genomic Analysis; I. Introduction; II. What Are RAPDs?; III. Advantages and Disadvantages of RAPDs as Polymorphic Markers
  • IV. Methods for Developing RAPD MarkersV. Using RAPDs to Identify Markers Closely Linked to a Mutation; VI. Conversion of RAPD Markers to Sequence Tagged Sites; VII. Conclusions; References; Chapter 10. Simple Sequence-Length Polymorphism Analysis; I. Analysis in DNA Sequencing Gels; II. Alternative Analysis of SSLP-Containing PCR Product in High-Resolution Agarose Gels; III. Troubleshooting and Other Considerations; References; Chapter 11. Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis; I. Introduction; II. SSCP Methodology; III. Gene Mapping; IV. Methods

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