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Vectorial transport of proteins into and across membranes /

This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. This volume, Vectorial Transport of Proteins into and across Membranes, brings togethe...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Tartakoff, Alan Michael
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1991.
Colección:Methods in cell biology ; v. 34.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Use of antiidiotype antibodies for the characterization of protein-protein interactions
  • In vivo protein translocation into or across the bacterial plasma membrane
  • Analysis of membrane protein topology using alkaline Phosphatase and [beta]-Galactosidase gene fusions
  • Expression of foreign polypeptides at the Escherichia coli cell surface
  • In vitro biochemical studies on translocation of presecretory proteins across the cytoplasmic membrane of Escherichia coli
  • Preprotein translocase of Escherichia coli
  • In vitro protein translocation into Escherichia coli inverted membrane vesicles
  • Membrane components of the protein secretion machinery
  • Signal sequence-independent protein secretion in gram-negative bacteria
  • Transcription of full-length and truncated mRNA transcripts to study protein translocation across the endoplasmic reticulum
  • Probing the molecular environment of translocating polypeptide chains by cross-linking.
  • Reconstitution of secretory protein translocation from detergent-solubilized rough microsomes
  • Analysis of protein topology in the endoplasmic reticulum
  • Protein import into peroxisomes in vitro
  • In vitro reconstitution of protein transport into chloroplasts
  • Analysis of mitochondrial protein import using translocation intermediates and specific antibodies
  • Import of precursor proteins into yeast submitochondrial particles
  • Pulse labeling of yeast cells as a tool to study mitochondrial protein import
  • Protein import machinery of yeast mitochondria
  • Protein import into isolated yeast mitochondria
  • Mitochondrial inner membrane protease I of Saccharomyces cerevisiae
  • Purified precursor proteins for studying protein import into yeast mitochondria
  • Cross-linking reagents as tools for identifying components of the yeast mitochondrial protein import machinery.