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Microbes as tools for cell biology /
Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor Corporativo: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in cell biology ;
v. 45. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; Microbes as Tools for Cell Biology; Copyright Page; Contents; Contributors; Preface; Introduction; Chapter 1. Obtaining and Maintaining Microbial Pathogens; I. Introduction; II. Biosafety Considerations; III. Department of Health and Human Services Guidelines; IV. Federal and Institutional Requirements; V. Source of Pathogens; VI. Culture and Maintenance of Pathogens; VII. Conclusion; Part I: Manipulation of Pathogens; Chapter 2. Cultivation of Malaria Parasites; I. Introduction; II. Culture Medium; III. Serum; IV. Erythrocytes; V. Culture Systems; VI. Synchronization.
- VII. Gametocyte Production in CultureVIII. Cloning Methods; IX. Cryopreservation; X. Serum Replacement; XI. Axenic (Extracellular) Development of Erythrocytic Stages of P. falciparum; XII. Cultivation of Erythrocytic Stages of Other Species of Malaria Parasites; XIII. In Vitro Development of Preerythrocytic and Sporogonic Stages of the Life Cycle; References; Chapter 3. Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii; I. Introductory Overview; II. In Vitro Culture of T. gondii Tachyzoites; III. Molecular Transformation Systems for Toxoplasma.
- IV. Summary and OutlookReferences; Chapter 4. Transfection Experiments with Leishmania; I. Introduction; II. Stable and Transient Transfection; III. Transfection; References; Chapter 5. Mutagenesis and Variant Selection in Salmonella; I. Introduction; II. Mutagenesis; III. Screening of Variants; IV. Genetic Analysis; V. Perspectives; References; Chapter 6. Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection; I. Introduction; II. Biosafety Considerations; III. Culture Media and Conditions; IV. Isolation of Mycobacteria; V. Maintenance of Stocks; VI. Genetic Techniques.
- VII. MutagenesisVIII. Strain Construction; IX. Mutant Selection and Isolation; References; Part II: Microbial Adherence and Invasion Assays; Chapter 7. Modulation of Murine Macrophage Behavior in Vivo and in Vitro; I. Introduction; II. Reagents and Solutions; III. Animal Husbandry and Maintenance of Immunocompromised Mice; IV. Eliciting and Harvesting Macrophages in Vivo; V. Adherence of Macrophages to Solid Substrates; VI. Assays for Macrophage Phagocytic Activity; VII. Analysis of MHC Class II Antigen Expression; References.
- Chapter 8. In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal TransductionI. Introduction; II. Phagocytosis Assays; III. Phagocytic Receptors; IV. Stimulation of Phagocytosis; V. Phagosome Isolation; References; Chapter 9. Bacterial Adhesion and Colonization Assays; I. Introduction; II. In Situ Screening of Host Receptor Distribution; III. Bacterial Adherence to Cells in Culture; IV. Biochemical Characterization of the Molecular Nature of Receptors in Situ; V. Bacterial Inhibition Experiments in Situ.