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Microbes as tools for cell biology /
Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor Corporativo: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in cell biology ;
v. 45. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Microbes as tools for cell biology / |c edited by David G. Russell. |
| 260 | |a San Diego : |b Academic Press, |c �1994. | ||
| 300 | |a 1 online resource (xvi, 339 pages) : |b illustrations | ||
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| 490 | 1 | |a Methods in cell biology ; |v v. 45 | |
| 500 | |a "Prepared under the auspices of the American Society for Cell Biology." | ||
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents. Key Features * Introduction by renowned microbiologist Dr. Stanley Falkow * Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes * Guides researchers in the study of intracellular pathogenesis * Describes microbial adherence and phagocytosis assays * Focuses on protein trafficking in infected cells * Well-illustrated with color plates, halftones, and diagrams. | ||
| 505 | 0 | |a Front Cover; Microbes as Tools for Cell Biology; Copyright Page; Contents; Contributors; Preface; Introduction; Chapter 1. Obtaining and Maintaining Microbial Pathogens; I. Introduction; II. Biosafety Considerations; III. Department of Health and Human Services Guidelines; IV. Federal and Institutional Requirements; V. Source of Pathogens; VI. Culture and Maintenance of Pathogens; VII. Conclusion; Part I: Manipulation of Pathogens; Chapter 2. Cultivation of Malaria Parasites; I. Introduction; II. Culture Medium; III. Serum; IV. Erythrocytes; V. Culture Systems; VI. Synchronization. | |
| 505 | 8 | |a VII. Gametocyte Production in CultureVIII. Cloning Methods; IX. Cryopreservation; X. Serum Replacement; XI. Axenic (Extracellular) Development of Erythrocytic Stages of P. falciparum; XII. Cultivation of Erythrocytic Stages of Other Species of Malaria Parasites; XIII. In Vitro Development of Preerythrocytic and Sporogonic Stages of the Life Cycle; References; Chapter 3. Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii; I. Introductory Overview; II. In Vitro Culture of T. gondii Tachyzoites; III. Molecular Transformation Systems for Toxoplasma. | |
| 505 | 8 | |a IV. Summary and OutlookReferences; Chapter 4. Transfection Experiments with Leishmania; I. Introduction; II. Stable and Transient Transfection; III. Transfection; References; Chapter 5. Mutagenesis and Variant Selection in Salmonella; I. Introduction; II. Mutagenesis; III. Screening of Variants; IV. Genetic Analysis; V. Perspectives; References; Chapter 6. Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection; I. Introduction; II. Biosafety Considerations; III. Culture Media and Conditions; IV. Isolation of Mycobacteria; V. Maintenance of Stocks; VI. Genetic Techniques. | |
| 505 | 8 | |a VII. MutagenesisVIII. Strain Construction; IX. Mutant Selection and Isolation; References; Part II: Microbial Adherence and Invasion Assays; Chapter 7. Modulation of Murine Macrophage Behavior in Vivo and in Vitro; I. Introduction; II. Reagents and Solutions; III. Animal Husbandry and Maintenance of Immunocompromised Mice; IV. Eliciting and Harvesting Macrophages in Vivo; V. Adherence of Macrophages to Solid Substrates; VI. Assays for Macrophage Phagocytic Activity; VII. Analysis of MHC Class II Antigen Expression; References. | |
| 505 | 8 | |a Chapter 8. In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal TransductionI. Introduction; II. Phagocytosis Assays; III. Phagocytic Receptors; IV. Stimulation of Phagocytosis; V. Phagosome Isolation; References; Chapter 9. Bacterial Adhesion and Colonization Assays; I. Introduction; II. In Situ Screening of Host Receptor Distribution; III. Bacterial Adherence to Cells in Culture; IV. Biochemical Characterization of the Molecular Nature of Receptors in Situ; V. Bacterial Inhibition Experiments in Situ. | |
| 650 | 0 | |a Microbiology. | |
| 650 | 0 | |a Cytology. | |
| 650 | 0 | |a Medical microbiology. | |
| 650 | 0 | |a Bacteriology |x Technique. | |
| 650 | 0 | |a Bacteria |x Adhesion. | |
| 650 | 0 | |a Pathogenic microorganisms. | |
| 650 | 0 | |a Host-bacteria relationships. | |
| 650 | 0 | |a Host-parasite relationships. | |
| 650 | 0 | |a Intracellular pathogens. | |
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| 650 | 0 | |a Microbiology |x Technique. | |
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